Human MMP-3 (Matrix Metalloproteinase 3) CLIA Kit (E-CL-H0931)

Name: Human MMP-3 (Matrix Metalloproteinase 3) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0931
Price: 180.0 USD
Availability: InStock

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Human MMP-3 (Matrix Metalloproteinase 3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MMP-3 in samples.No significant cross-reactivity or interference between Human MMP-3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56010.000	62748.000	59379.000	59340
1000	23320.000	25226.000	24273.000	24234
500	12430.000	10658.000	11544.000	11505
250	5536.000	6072.000	5804.000	5765
125	2808.000	2458.000	2633.000	2594
62.5	1396.000	1392.000	1394.000	1355
31.25	691.000	727.000	709.000	670
0	37.000	41.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	106.700	311.190	682.010	111.190	301.410	673.140
Standard deviation	9.020	30.530	48.970	10.160	22.360	74.920
CV（%）	8.450	9.810	7.180	9.140	7.420	11.130

Recovery

The recovery of Human MMP-3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	101-113	107
EDTA plasma （n=8）	87-98	93
Cell culture media （n=8）	87-100	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MMP-3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-104	98-112	95-110
1:2	Average	98	105	103
1:4	Range	96-112	88-99	95-110
1:4	Average	102	94	101
1:8	Range	103-116	94-106	103-120
1:8	Average	110	99	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MMP-3. You can calculate the concentration of Human MMP-3 in the samples by comparing the RLU value of the samples to the standard curve.

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