|Detection range||15.63-1000 pg/mL|
|Sample type &Sample volume||serum, plasma and other biological fluids; 50μL|
|Specificity||This kit recognizes MT in samples. No significant cross-reactivity or interference between MT and analogues was observed.|
|Reproducibility||Both intra-CV and inter-CV are < 10%.|
|Application||This ELISA kit applies to the in vitro quantitative determination of MT concentrations in serum, plasma and other biological fluids.|
|Micro ELISA Plate(Dismountable)||96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
|-20℃, 6 months|
|Reference Standard||96T: 2 vials
48T: 1 vial
|Concentrated Biotinylated Detection Ab (100×)||96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
|Concentrated HRP Conjugate (100×)||96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
|-20℃(Protect from light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||2-8°C, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25×)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||2-8℃(Protect from light)|
|Stop Solution||1 vial, 10 mL||2-8℃|
|Plate Sealer||5 pieces|
|Certificate of Analysis||1 copy|
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MT were tested on 3 different plates, 20 replicates in each plate.
|Intra-assay Precision||Inter-assay Precision|
The recovery of MT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
|Sample Type||Range (%)||Average Recovery (%)|
|Cell culture media(n=8)||89-103||96|
Samples were spiked with high concentrations of Human MT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results
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|Sample: Cell lysate|
|Sample: Tissue homogenate|
j****tSubmitted [ Oct 02 2021 ]
Asked: hello i used human melatonin elisa kit for anlaysing salivary melatonin levels, i got the result values from 10 to 999, is there any mistake i made and how to interpret my results?
adminSubmitted [ Oct 02 2021 ]
Answered: Hi Juliet, thanks for your feedback, In order to help you analysis the result, can you help to fill in the after-sales-form and send us back? I will send the and the after-sales-form to your email, pls kindly help to check it.
s***********iSubmitted [ Jan 24 2021 ]
Asked: I use horse serum for induction serum shock in my cell line culture and I wonder to know that is there any cross-reactivity in the detection of melatonin by your kit between horse serum melatonin and human cell line supernatant melatonin
adminSubmitted [ Jan 24 2021 ]
Answered: MT(melatonin) is a small molecule with almost no difference in structure among different species. We suppose that there is cross-reactivity in the detection of melatonin between horse serum melatonin and human cell line supernatant melatonin.