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Human MT(Melatonin) ELISA Kit

  • Cat.No.:E-EL-H2016

  • Reactivity: Human

To Purchase E-EL-H2016

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes MT in samples. No significant cross-reactivity or interference between MT and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of MT concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MT were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 45.90 151.70 400.20 43.50 161.20 418.60
Standard deviation 2.50 7.90 20.80 2.30 6.90 23.00
CV (%) 5.45 5.21 5.20 5.29 4.28 5.49


The recovery of MT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-109 102
EDTA plasma(n=8) 92-106 98
Cell culture media(n=8) 89-103 96


Samples were spiked with high concentrations of Human MT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaMetabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


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    Measuring melatonin by immunoassay

    DOI: 10.1111/jpi.12657

    Sample: serum,plasma,biological fluids
  2. Clinical and Translational Medicine (2022) IF: 11.492
    Melatonin enhances osteoblastogenesis of senescent bone marrow stromal cells through NSD2-mediated chromatin remodelling

    DOI: 10.1002/ctm2.746

    Sample: bone marrow plasma
    Increased Expression of Colonic Mucosal Melatonin in Patients with Irritable Bowel Syndrome Correlated with Gut Dysbiosis

    DOI: 10.1016/j.gpb.2020.06.013

    PMID: 33607299

    Sample: Cell culture supernatant(BON-1 cells)
  4. Frontiers in Nutrition (2022) IF: 6.59
    Sleep duration of lactating mothers and its relationship with feeding pattern, milk macronutrients and related serum factors: A combined longitudinal cohort and cross-sectional study

    DOI: 10.3389/fnut.2022.973291

    PMID: 36110402

    Sample: plasma
  5. Cells (2022) IF: 6.6
    Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

    DOI: 10.3390/cells11101690

    PMID: 35626727

    Sample: serum
  6. Nutrients (2021) IF: 5.719
    Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway

    DOI: 10.3390/nu14010117

    PMID: 35010992

    Sample: BON-1 cell
  7. Frontiers in Aging Neuroscience (2021) IF: 5.75
    Peripheral Clock System Abnormalities in Patients With Parkinson’s Disease

    DOI: 10.3389/fnagi.2021.736026

    Sample: plasma
  8. MITOCHONDRION (2021) IF: 4.16
    Yoga improves mitochondrial health and reduces severity of autoimmune inflammatory arthritis: A randomized controlled trial

    DOI: 10.1016/j.mito.2021.03.004

    PMID: 33741520

    Sample: serum
  9. Aging-US (2020) IF: 4.831
    Melatonin exerts immunoregulatory effects by balancing peripheral effector and regulatory T helper cells in myasthenia gravis

    DOI: 10.18632/aging.103785

    PMID: 33136553

    Sample: Serum
  10. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients

    DOI: 10.3390/ijerph18094852

    PMID: 34062830

    Sample: Serum
    Melatonin regulates proliferation, apoptosis and invasion of trophoblasts in preeclampsia by inhibiting endoplasmic reticulum stress

    DOI: 10.1111/aji.13585

    PMID: 35716034

    Sample: serum
    Peculiarities of melatonin levels in preterm infants

    DOI: 10.1007/s00508-022-02109-9

    Sample: urine
  13. METABOLIC BRAIN DISEASE (2019) IF: 2.411
    Evidence for an association of serum melatonin concentrations with recognition and circadian preferences in patients with schizophrenia

    DOI: 10.1007/s11011-019-00395-3

    PMID: 30758707

    Sample: Serum
  14. Laryngoscope Investigative Otolaryngology (2021) IF: 2.458
    Relationship between physical activity, objective sleep parameters, and circadian rhythm in patients with head and neck cancer receiving chemoradiotherapy: A longitudinal study

    DOI: 10.1002/lio2.664

    Sample: Urine
  15. EPILEPSY & BEHAVIOR (2020) IF: 2.508
    The relationship between epileptic seizure and melatonin in children

    DOI: 10.1016/j.yebeh.2020.107345

    Sample: blood
  16. CHILDS NERVOUS SYSTEM (2022) IF: 1.532
    Temporal profile of serum melatonin levels in paediatric pineal tumours subjected to surgery: newer insights

    DOI: 10.1007/s00381-022-05601-9

    PMID: 36045301

    Sample: serum
  17. PHYCOLOGIA (2019) IF: 1.976
    Exogenous melatonin confers salt stress tolerance to Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) by improving redox homeostasis

    DOI: 10.2216/17-141.1

    Sample: Cell culture supernatant
  18. PHYCOLOGIA (2019) IF: 1.976
    Enhanced melatonin production via aralkylamine N-acetyltransferase overexpression enhances NaCl resistance in transgenic Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)

    DOI: 10.1080/00318884.2018.1529971

    Sample: Tissue homogenate
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  • Q&A

a********lSubmitted [ Aug 02 2023 ]

Asked: I used this kit I want to learn the intra- and inter-assay coefficients of variation were ..........% at the range of, respectively. The mean recovery of melatonin was .... %, and the sensitivity of the assay was ... pg/mL. for my study could you help me



adminSubmitted [ Aug 02 2023 ]

Answered: For E-EL-H2016, the intra- and inter-assay coefficients of variation were 5.20-5.45% at the range of 45.9-400.20pg/mL and 4.28-5.49% at the range of 43.50-418.60 pg/mL, respectively. The mean serum, EDTA plasma and cell culture media recovery of melatonin was 102%, 98%,96% and the sensitivity of the assay was 9.38 pg/mL.For details, I will reply you by email. Please pay attention to your email


adminSubmitted [ Aug 02 2023 ]

Answered: For E-EL-H2016, the intra- and inter-assay coefficients of variation were 5.20-5.45% at the range of 45.9-400.20pg/mL and 4.28-5.49% at the range of 43.50-418.60 pg/mL, respectively. The mean serum, EDTA plasma and cell culture media recovery of melatonin was 102%, 98%,96% and the sensitivity of the assay was 9.38 pg/mL. For details, I will reply you by email. Please pay attention to your email


M***** Submitted [ May 23 2023 ]

Asked: How many test can be done with one kit, or we require one kit for one person



adminSubmitted [ May 23 2023 ]

Answered: Thanks for your support.We will reply you by E-mail.


j****tSubmitted [ Oct 02 2021 ]

Asked: hello i used human melatonin elisa kit for anlaysing salivary melatonin levels, i got the result values from 10 to 999, is there any mistake i made and how to interpret my results?



adminSubmitted [ Oct 02 2021 ]

Answered: Hi Juliet, thanks for your feedback, In order to help you analysis the result, can you help to fill in the after-sales-form and send us back? I will send the and the after-sales-form to your email, pls kindly help to check it.


s***********iSubmitted [ Jan 24 2021 ]

Asked: I use horse serum for induction serum shock in my cell line culture and I wonder to know that is there any cross-reactivity in the detection of melatonin by your kit between horse serum melatonin and human cell line supernatant melatonin



adminSubmitted [ Jan 24 2021 ]

Answered: MT(melatonin) is a small molecule with almost no difference in structure among different species. We suppose that there is cross-reactivity in the detection of melatonin between horse serum melatonin and human cell line supernatant melatonin.

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