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Human MT(Melatonin) ELISA Kit

  • Cat.No.:E-EL-H2016

  • Reactivity: Human

To Purchase E-EL-H2016

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes MT in samples. No significant cross-reactivity or interference between MT and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of MT concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MT were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 45.90 151.70 400.20 43.50 161.20 418.60
Standard deviation 2.50 7.90 20.80 2.30 6.90 23.00
CV (%) 5.45 5.21 5.20 5.29 4.28 5.49


The recovery of MT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-109 102
EDTA plasma(n=8) 92-106 98
Cell culture media(n=8) 89-103 96


Samples were spiked with high concentrations of Human MT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaMetabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Metabolic brain disease (2019) IF: 2.441
    Evidence for an association of Serum melatonin concentrations with recognition and circadian preferences in patients with schizophrenia

    DOI: 10.1007/s11011-019-00395-3

    PMID: 30758707

    Sample: Serum
  2. Phycologia (2019) IF: 1.798
    Enhanced melatonin production via aralkylamine N-acetyltransferase overexpression enhances NaCl resistance in transgenic Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)

    DOI: 10.1080/00318884.2018.1529971

    Sample: Cell lysate
  3. PHYCOLOGIA (2019) IF: 1.976
    Enhanced production of melatonin by overexpression of arylalkylamine N-acetyltransferase plays a role in NaCl resistance in transgenic Chlamydomonas

    DOI: 10.1080/00318884.2018.1529971

    Sample: Tissue homogenate
  4. Phycologia: 2018 (2018) IF: 1.798
    Exogenous melatonin confers salt stress tolerance to Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) by improving redox homeostasis.

    DOI: 10.2216/17-141.1

  5. Bezmialem Science (2020)
    Serum Melatonin Levels in Patients with Sudden Sensorineural Hearing Loss

    DOI: 10.14235/bas.galenos.2019.3459

    Sample: Serum
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  • Reviews
  • Q&A

j****tSubmitted [ Oct 02 2021 ]

Asked: hello i used human melatonin elisa kit for anlaysing salivary melatonin levels, i got the result values from 10 to 999, is there any mistake i made and how to interpret my results?



adminSubmitted [ Oct 02 2021 ]

Answered: Hi Juliet, thanks for your feedback, In order to help you analysis the result, can you help to fill in the after-sales-form and send us back? I will send the and the after-sales-form to your email, pls kindly help to check it.


s***********iSubmitted [ Jan 24 2021 ]

Asked: I use horse serum for induction serum shock in my cell line culture and I wonder to know that is there any cross-reactivity in the detection of melatonin by your kit between horse serum melatonin and human cell line supernatant melatonin



adminSubmitted [ Jan 24 2021 ]

Answered: MT(melatonin) is a small molecule with almost no difference in structure among different species. We suppose that there is cross-reactivity in the detection of melatonin between horse serum melatonin and human cell line supernatant melatonin.

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