Human MUC5AC (Mucin 5 Subtype AC) CLIA Kit (E-CL-H1340)

Name: Human MUC5AC (Mucin 5 Subtype AC) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1340
Price: 180.0 USD
Availability: InStock

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Human MUC5AC (Mucin 5 Subtype AC) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MUC5AC in samples.No significant cross-reactivity or interference between Human MUC5AC and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	57225.000	58567.000	57896.000	57869
500	21130.000	24152.000	22641.000	22614
250	11950.000	10336.000	11143.000	11116
125	5028.000	5690.000	5359.000	5332
62.5	2724.000	2402.000	2563.000	2536
31.25	1473.000	1309.000	1391.000	1364
15.63	693.000	743.000	718.000	691
0	27.000	27.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	53.620	107.890	352.390	52.640	114.800	336.150
Standard deviation	6.400	9.890	31.500	6.470	10.390	21.040
CV（%）	11.940	9.170	8.940	12.290	9.050	6.260

Recovery

The recovery of Human MUC5AC was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-114	104
EDTA plasma （n=8）	97-110	105
Cell culture media （n=8）	100-114	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MUC5AC and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-108	88-98	100-116
1:2	Average	102	93	106
1:4	Range	102-114	99-116	96-110
1:4	Average	108	106	104
1:8	Range	83-96	101-116	97-112
1:8	Average	90	106	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC5AC. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC5AC, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MUC5AC. You can calculate the concentration of Human MUC5AC in the samples by comparing the RLU value of the samples to the standard curve.

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