Human MYL9 (Myosin Light Chain 9, Regulatory) CLIA Kit (E-CL-H0524)

Name: Human MYL9 (Myosin Light Chain 9, Regulatory) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0524
Price: 180.0 USD
Availability: InStock

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Human MYL9 (Myosin Light Chain 9, Regulatory) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MYL9 in samples.No significant cross-reactivity or interference between Human MYL9 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	57852.000	58966.000	58409.000	58376
500	21933.000	23577.000	22755.000	22722
250	11629.000	10139.000	10884.000	10851
125	5294.000	5430.000	5362.000	5329
62.5	2756.000	2380.000	2568.000	2535
31.25	1421.000	1223.000	1322.000	1289
15.63	718.000	756.000	737.000	704
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	51.790	84.090	372.030	48.620	89.880	400.180
Standard deviation	5.960	6.250	27.120	5.840	8.390	38.100
CV（%）	11.510	7.430	7.290	12.010	9.330	9.520

Recovery

The recovery of Human MYL9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-108	100
EDTA plasma （n=8）	89-102	94
Cell culture media （n=8）	97-112	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MYL9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-98	93-110	98-113
1:2	Average	92	100	103
1:4	Range	84-97	100-116	86-100
1:4	Average	90	106	92
1:8	Range	95-110	94-109	95-108
1:8	Average	101	99	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYL9. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYL9, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MYL9. You can calculate the concentration of Human MYL9 in the samples by comparing the RLU value of the samples to the standard curve.

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