Human NE/ELA2 (Elastase 2, Neutrophil) CLIA Kit (E-CL-H1196)

Name: Human NE/ELA2 (Elastase 2, Neutrophil) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1196
Price: 180.0 USD
Availability: InStock

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Human NE/ELA2 (Elastase 2, Neutrophil) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NE/ELA2 in samples.No significant cross-reactivity or interference between Human NE/ELA2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	31802.000	37874.000	34838.000	34808
5	15533.000	16675.000	16104.000	16074
2.5	7843.000	7785.000	7814.000	7784
1.25	3768.000	3902.000	3835.000	3805
0.63	1884.000	1878.000	1881.000	1851
0.31	938.000	822.000	880.000	850
0.16	347.000	371.000	359.000	329
0	30.000	30.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.480	1.150	4.330	0.490	1.210	4.410
Standard deviation	0.040	0.090	0.270	0.050	0.090	0.410
CV（%）	8.330	7.830	6.240	10.200	7.440	9.300

Recovery

The recovery of Human NE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-108	103
EDTA plasma （n=8）	97-111	102
Cell culture media （n=8）	98-112	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-104	98-115	86-97
1:2	Average	97	105	92
1:4	Range	89-101	84-99	93-106
1:4	Average	94	91	99
1:8	Range	99-117	101-117	102-116
1:8	Average	107	109	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NE/ELA2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NE/ELA2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NE/ELA2. You can calculate the concentration of Human NE/ELA2 in the samples by comparing the RLU value of the samples to the standard curve.

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