Human NF-κB p65 (Nuclear Factor Kappa B p65) CLIA Kit (E-CL-H0902)
Add to cart
For research use only.
Product Summary
| Sensitivity | 18.75 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human NF-κB p65 in samples.No significant cross-reactivity or interference between Human NF-κB p65 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Chemiluminescence |
| Assay Type | Sandwich-CLIA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃/-20℃ |
| Expiration Date | 6 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
|
|||||||||||||||||||||||||||||||||||||||||||||
| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
|
|||||||||||||||||||||||||||||||||||||||||||||
| Recovery |
The recovery of Human NF-κBp65 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
|
|||||||||||||||||||||||||||||||||||||||||||||
| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human NF-κBp65 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
|
|||||||||||||||||||||||||||||||||||||||||||||
Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NF-κB p65. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NF-κB p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NF-κB p65, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NF-κB p65. You can calculate the concentration of Human NF-κB p65 in the samples by comparing the RLU value of the samples to the standard curve.
Background
| Gene ID | 5970 |
| Uniport ID | Q04206 |
| Research Area | Cancer , CellBiology , Cardiovascular , EpigeneticsandNuclearSignaling , Metabolism , Microbiology , Neuroscience , Signaltransduction |
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Videos
Resources
Contact with Us
Toll-free: 1-888-852-8623
Tel: 1-832-243-6086
Add:
14780 Memorial Drive, Suite 105,
Houston, Texas, 77079, USA
Houston, Texas, 77079, USA
