Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit (E-CL-H0541)

Name: Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0541
Price: 180.0 USD
Availability: InStock

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Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NOS2/iNOS in samples.No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56543.000	59401.000	57972.000	57937
1000	24159.000	26279.000	25219.000	25184
500	11873.000	10983.000	11428.000	11393
250	5640.000	5748.000	5694.000	5659
125	2753.000	2655.000	2704.000	2669
62.5	1380.000	1342.000	1361.000	1326
31.25	646.000	728.000	687.000	652
0	34.000	36.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	96.470	190.320	883.120	105.040	190.480	951.040
Standard deviation	8.800	14.390	55.280	8.450	16.040	65.810
CV（%）	9.120	7.560	6.260	8.040	8.420	6.920

Recovery

The recovery of Human NOS2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-100	95
EDTA plasma （n=8）	88-103	95
Cell culture media （n=8）	92-105	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NOS2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-115	88-101	89-101
1:2	Average	107	94	95
1:4	Range	90-104	99-114	89-105
1:4	Average	97	105	96
1:8	Range	92-107	96-109	103-118
1:8	Average	98	103	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NOS2/iNOS. You can calculate the concentration of Human NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.

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