Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit (E-CL-H0543)

Name: Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0543
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NOS3/eNOS in samples.No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	55819.000	60239.000	58029.000	58008
500	22384.000	22770.000	22577.000	22556
250	12138.000	10290.000	11214.000	11193
125	5068.000	5184.000	5126.000	5105
62.5	2643.000	2535.000	2589.000	2568
31.25	1346.000	1306.000	1326.000	1305
15.63	749.000	787.000	768.000	747
0	21.000	21.000	21.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	49.990	142.230	366.470	45.510	148.760	366.500
Standard deviation	6.460	14.220	22.320	5.180	13.920	29.720
CV（%）	12.920	10.000	6.090	11.380	9.360	8.110

Recovery

The recovery of Human NOS3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-102	97
EDTA plasma （n=8）	98-116	106
Cell culture media （n=8）	90-105	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NOS3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-102	101-113	89-102
1:2	Average	96	107	96
1:4	Range	87-99	102-115	89-103
1:4	Average	94	108	97
1:8	Range	85-95	100-116	99-116
1:8	Average	90	107	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS3/eNOS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NOS3/eNOS. You can calculate the concentration of Human NOS3/eNOS in the samples by comparing the RLU value of the samples to the standard curve.

Background