Human NSE (Neuron-Specific Enolase) CLIA Kit (E-CL-H0702)

Name: Human NSE (Neuron-Specific Enolase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0702
Price: 180.0 USD
Availability: InStock

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Human NSE (Neuron-Specific Enolase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.28 ng/mL
Detection Range	0.47-30 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NSE in samples.No significant cross-reactivity or interference between Human NSE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
30	39007.000	39021.000	39014.000	38984
15	13182.000	15632.000	14407.000	14377
7.5	6343.000	5963.000	6153.000	6123
3.75	2859.000	3087.000	2973.000	2943
1.88	1665.000	1621.000	1643.000	1613
0.94	1086.000	930.000	1008.000	978
0.47	671.000	703.000	687.000	657
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.490	4.370	10.890	1.350	4.660	11.500
Standard deviation	0.190	0.400	1.210	0.170	0.490	0.700
CV（%）	12.750	9.150	11.110	12.590	10.520	6.090

Recovery

The recovery of Human NSE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-117	107
EDTA plasma （n=8）	95-110	100
Cell culture media （n=8）	89-102	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NSE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-107	99-112	93-103
1:2	Average	101	106	98
1:4	Range	96-110	96-107	86-101
1:4	Average	103	102	92
1:8	Range	94-111	101-117	91-106
1:8	Average	101	109	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NSE. You can calculate the concentration of Human NSE in the samples by comparing the RLU value of the samples to the standard curve.

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