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Human NSE (Neuron-Specific Enolase) CLIA Kit

Cat:E-CL-H0702
Manual MSDS

Price: $ 682

Price: $ 546

Price: $ 180

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Price: Inquire

Size:
96T 48T 24T 96T*10 96T*5
Quantity:
  • Reactivity: Human
  • Detection Range: 0.47-30 ng/mL
  • Sensitivity: 0.28 ng/mL
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Product Details
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NSE. You can calculate the concentration of Human NSE in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type Sandwich-ELISA
size 96T / 48T / 24T / 96T*10 / 96T*5
Assay Time 3.5h
Detection Method Chemiluminescence
Detection Range 0.47-30 ng/mL
Sensitivity 0.28 ng/mL
Sample Volume 100μL
Sample Type serum, plasma and other biological fluids
Specificity This kit recognizes Human NSE in samples.No significant cross-reactivity or interference between Human NSE and analogues was observed
Precision Both intra-CV and inter-CV are < 10%.
Recovery 80%-120%
Introduction This ELISA kit applies to the in vitro quantitative determination of Human NSE concentrations in serum, plasma and other biological fluids.
Applications CLIA
Storage 2-8℃/-20℃
Database SwissProt:  P09104
Syonoyms Enolase γ,Enolase 2
Research Area Cancer Metabolism Developmental Biology Neuroscience Stem Cells Tags And Cell Markers