Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit (E-CL-H0620)

Name: Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0620
Price: 180.0 USD
Availability: InStock

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Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NT-proBNP in samples.No significant cross-reactivity or interference between Human NT-proBNP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	51605.000	58969.000	55287.000	55253
500	21645.000	26345.000	23995.000	23961
250	10262.000	10214.000	10238.000	10204
125	4825.000	5485.000	5155.000	5121
62.5	2583.000	2417.000	2500.000	2466
31.25	1428.000	1382.000	1405.000	1371
15.63	724.000	816.000	770.000	736
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.100	155.350	408.420	46.440	148.560	430.490
Standard deviation	4.700	13.340	43.700	4.610	14.260	33.490
CV（%）	9.980	8.590	10.700	9.930	9.600	7.780

Recovery

The recovery of Human NT-proBNP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-96	91
EDTA plasma （n=8）	102-116	108
Cell culture media （n=8）	94-112	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NT-proBNP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-107	87-98	93-108
1:2	Average	99	92	100
1:4	Range	96-113	96-114	96-110
1:4	Average	104	104	102
1:8	Range	92-108	94-112	96-113
1:8	Average	99	102	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proBNP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NT-proBNP. You can calculate the concentration of Human NT-proBNP in the samples by comparing the RLU value of the samples to the standard curve.

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