Human OCLN (Occludin) CLIA Kit (E-CL-H0720)

Name: Human OCLN (Occludin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0720
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human OCLN in samples.No significant cross-reactivity or interference between Human OCLN and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55560.000	64150.000	59855.000	59831
1000	24285.000	27207.000	25746.000	25722
500	12073.000	12015.000	12044.000	12020
250	5565.000	5977.000	5771.000	5747
125	2930.000	2724.000	2827.000	2803
62.5	1474.000	1370.000	1422.000	1398
31.25	644.000	736.000	690.000	666
0	23.000	25.000	24.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	109.150	195.210	975.230	117.290	191.990	954.490
Standard deviation	13.070	22.160	104.840	11.710	22.310	87.810
CV（%）	11.970	11.350	10.750	9.980	11.620	9.200

Recovery

The recovery of Human OCLN was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-108	101
EDTA plasma （n=8）	90-105	96
Cell culture media （n=8）	99-112	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human OCLN and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-101	97-112	101-119
1:2	Average	96	103	109
1:4	Range	88-99	98-114	103-119
1:4	Average	93	106	109
1:8	Range	89-102	85-99	97-113
1:8	Average	95	90	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OCLN. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OCLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OCLN, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human OCLN. You can calculate the concentration of Human OCLN in the samples by comparing the RLU value of the samples to the standard curve.

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