Human OPN (Osteopontin) CLIA Kit (E-CL-H0885)

Name: Human OPN (Osteopontin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0885
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human OPN in samples.No significant cross-reactivity or interference between Human OPN and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	61099.000	66005.000	63552.000	63529
2000	27032.000	30982.000	29007.000	28984
1000	14339.000	12839.000	13589.000	13566
500	6816.000	6990.000	6903.000	6880
250	3466.000	3036.000	3251.000	3228
125	1705.000	1681.000	1693.000	1670
62.5	808.000	832.000	820.000	797
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	209.130	490.860	1620.530	189.290	459.490	1517.490
Standard deviation	19.260	54.730	122.670	18.650	49.620	148.260
CV（%）	9.210	11.150	7.570	9.850	10.800	9.770

Recovery

The recovery of Human OPN was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-100	92
EDTA plasma （n=8）	92-105	97
Cell culture media （n=8）	89-101	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human OPN and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-99	102-120	95-109
1:2	Average	91	110	101
1:4	Range	99-111	93-108	85-98
1:4	Average	105	101	90
1:8	Range	102-118	90-104	98-113
1:8	Average	108	96	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OPN. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human OPN. You can calculate the concentration of Human OPN in the samples by comparing the RLU value of the samples to the standard curve.

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