Human ORM2 (Orosomucoid 2) CLIA Kit (E-CL-H1058)

Name: Human ORM2 (Orosomucoid 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1058
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ORM2 in samples.No significant cross-reactivity or interference between Human ORM2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	53620.000	54508.000	54064.000	54025
500	24147.000	24759.000	24453.000	24414
250	11454.000	10300.000	10877.000	10838
125	4920.000	5588.000	5254.000	5215
62.5	2729.000	2597.000	2663.000	2624
31.25	1416.000	1394.000	1405.000	1366
15.63	765.000	791.000	778.000	739
0	39.000	39.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	48.030	111.450	464.810	48.000	102.910	478.420
Standard deviation	5.860	9.270	43.920	4.320	10.250	53.870
CV（%）	12.200	8.320	9.450	9.000	9.960	11.260

Recovery

The recovery of Human ORM2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-110	102
EDTA plasma （n=8）	97-115	105
Cell culture media （n=8）	86-101	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ORM2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-115	95-112	89-105
1:2	Average	107	103	96
1:4	Range	89-102	83-95	95-108
1:4	Average	96	90	101
1:8	Range	91-102	102-118	103-118
1:8	Average	96	108	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ORM2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ORM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ORM2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ORM2. You can calculate the concentration of Human ORM2 in the samples by comparing the RLU value of the samples to the standard curve.

Background