Human P-Cadherin (Cadherin, Placental) CLIA Kit (E-CL-H0624)

Name: Human P-Cadherin (Cadherin, Placental) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0624
Price: 180.0 USD
Availability: InStock

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Human P-Cadherin (Cadherin, Placental) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human P-Cadherin in samples.No significant cross-reactivity or interference between Human P-Cadherin and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	50949.000	61731.000	56340.000	56308
500	20978.000	24260.000	22619.000	22587
250	11741.000	10035.000	10888.000	10856
125	4762.000	5364.000	5063.000	5031
62.5	2845.000	2489.000	2667.000	2635
31.25	1380.000	1196.000	1288.000	1256
15.63	695.000	759.000	727.000	695
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	52.300	132.830	408.680	56.360	141.500	442.940
Standard deviation	4.690	10.800	25.090	5.280	9.920	27.640
CV（%）	8.970	8.130	6.140	9.370	7.010	6.240

Recovery

The recovery of Human P-Cadherin was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-116	107
EDTA plasma （n=8）	91-106	98
Cell culture media （n=8）	98-112	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human P-Cadherin and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-98	98-114	89-103
1:2	Average	92	105	95
1:4	Range	88-100	99-114	92-107
1:4	Average	93	106	99
1:8	Range	97-109	93-108	86-102
1:8	Average	103	101	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human P-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human P-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human P-Cadherin. You can calculate the concentration of Human P-Cadherin in the samples by comparing the RLU value of the samples to the standard curve.

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