Human PAR2 (Protease Activated Receptor 2) CLIA Kit (E-CL-H0783)

Name: Human PAR2 (Protease Activated Receptor 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0783
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human PAR2 (Protease Activated Receptor 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PAR2 in samples.No significant cross-reactivity or interference between Human PAR2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54497.000	61237.000	57867.000	57835
1000	24084.000	28614.000	26349.000	26317
500	11636.000	10542.000	11089.000	11057
250	5338.000	5894.000	5616.000	5584
125	3050.000	2608.000	2829.000	2797
62.5	1494.000	1330.000	1412.000	1380
31.25	712.000	736.000	724.000	692
0	32.000	32.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	99.880	283.290	848.510	107.060	289.740	828.490
Standard deviation	10.720	22.380	64.830	12.580	21.150	50.790
CV（%）	10.730	7.900	7.640	11.750	7.300	6.130

Recovery

The recovery of Human PAR2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-115	105
EDTA plasma （n=8）	93-107	100
Cell culture media （n=8）	95-112	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PAR2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-110	101-117	93-104
1:2	Average	103	108	98
1:4	Range	96-110	88-100	100-116
1:4	Average	104	93	108
1:8	Range	86-97	93-104	95-107
1:8	Average	91	98	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PAR2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAR2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PAR2. You can calculate the concentration of Human PAR2 in the samples by comparing the RLU value of the samples to the standard curve.