Human PGR (Progesterone Receptor) CLIA Kit (E-CL-H1337)

Name: Human PGR (Progesterone Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1337
Price: 180.0 USD
Availability: InStock

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Human PGR (Progesterone Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PGR in samples.No significant cross-reactivity or interference between Human PGR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	60332.000	66856.000	63594.000	63572
2000	25833.000	30911.000	28372.000	28350
1000	15486.000	14136.000	14811.000	14789
500	6281.000	7153.000	6717.000	6695
250	3442.000	3154.000	3298.000	3276
125	1717.000	1483.000	1600.000	1578
62.5	759.000	797.000	778.000	756
0	22.000	22.000	22.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	216.020	574.030	1511.730	215.940	556.810	1426.170
Standard deviation	18.530	42.480	173.400	20.380	54.790	97.120
CV（%）	8.580	7.400	11.470	9.440	9.840	6.810

Recovery

The recovery of Human PGR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	102-114	108
EDTA plasma （n=8）	90-105	96
Cell culture media （n=8）	88-100	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PGR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-107	97-114	90-105
1:2	Average	97	105	96
1:4	Range	86-101	86-99	84-96
1:4	Average	93	93	91
1:8	Range	85-97	104-120	93-103
1:8	Average	90	110	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PGR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PGR. You can calculate the concentration of Human PGR in the samples by comparing the RLU value of the samples to the standard curve.

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