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Human PⅢNP(N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit

Cat.No.:E-EL-H0183
Reactivity: Human

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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	23.44-1500 pg/mL
Sensitivity	14.06 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human PⅢNP in samples. No significant cross-reactivity or interference between Human PⅢNP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human PⅢNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅢNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅢNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅢNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅢNP. You can calculate the concentration of Human PⅢNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PⅢNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PⅢNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	75.40	208.10	518.30	69.80	205.90	509.50
Standard deviation	4.80	11.40	18.70	3.90	9.90	18.30
CV (%)	6.37	5.48	3.61	5.59	4.81	3.59

Recovery

The recovery of Human PⅢNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-107	101
EDTA plasma (n=8)	96-107	102
Cell culture media (n=8)	85-99	92

Linearity

Samples were spiked with high concentrations of Human PⅢNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide
Research Area	Signal Transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BMC Medicine (2016) IF: 8.0050
No effects of oral vitamin D supplementation on non-alcoholic fatty liver disease in patients with type 2 diabetes: a randomized, double-blind, placebo-controlled trial

DOI: 10.1186/s12916-016-0638-y

Sample: Serum
JOURNAL OF CARDIOVASCULAR MAGNETIC RESONANCE (2020) IF: 5.361
Imaging, biomarker and invasive assessment of diffuse left ventricular myocardial fibrosis in atrial fibrillation

DOI: 10.1186/s12968-020-0603-y

PMID: 32036784
Sample: Whole Blood
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY (2022) IF: 4.114
Micro-fragmented adipose tissue (mFAT) associated with arthroscopic debridement provides functional improvement in knee osteoarthritis: a randomized controlled trial

DOI: 10.1007/s00167-022-07101-4

PMID: 36040510
Sample: serum
OSTEOARTHRITIS AND CARTILAGE (2017) IF: 4.742
Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis

DOI: 10.1016/j.joca.2017.05.015

Sample: Serum
PLoS One (2021) IF: 3.24
Lumenato protects normal human dermal fibroblasts from neutrophil-induced collagen-3 damage in co-cultures

DOI: 10.1371/journal.pone.0248183

PMID: 33730073
Sample: Cell culture supernatant
Frontiers in Physiology (2020) IF: 3.367
Elevated β1-Adrenergic Receptor Autoantibody Levels Increase Atrial Fibrillation Susceptibility by Promoting Atrial Fibrosis

DOI: 10.3389/fphys.2020.00076

Sample: Serum
WORLD JOURNAL OF GASTROENTEROLOGY (2019) IF: 3.411
On-treatment monitoring of liver fibrosis with serum hepatitis B core-related antigen in chronic hepatitis B

DOI: 10.3748/wjg.v25.i32.4764

PMID: 31528100
Sample: Serum
ESC Heart Failure (2022) IF: 3.612
Direct actions of dapagliflozin and interactions with LCZ696 and spironolactone on cardiac fibroblasts of patients with heart failure and reduced ejection fraction

DOI: 10.1002/ehf2.14186

PMID: 36303443
Sample: Cardiac fibroblasts
Diagnostics (2022) IF: 3.706
The Role of Circulating Collagen Turnover Biomarkers and Late Gadolinium Enhancement in Patients with Non-Ischemic Dilated Cardiomyopathy

DOI: 10.3390/diagnostics12061435

PMID: 35741245
Sample: serum
Annals of Intensive Care (2018) IF: 3.771
Driving pressure and long-term outcomes in moderate/severe acute respiratory distress syndrome

DOI: 10.1186/s13613-018-0469-4

PMID: 30535520
Sample: Serum
DIABETES-METABOLISM RESEARCH AND REVIEWS (2018) IF: 3.904
Procollagen‐III peptide identifies adipose tissue‐associated inflammation in type 2 diabetes with or without nonalcoholic liver disease

DOI: 10.1002/dmrr.2998

Sample: Serum
Pituitary (2020) IF: 3.954
The evaluation of the tendon and muscle changes of lower extremity in patients with acromegaly

DOI: 10.1007/s11102-020-01037-z

Sample: serum
HEART AND VESSELS (2015) IF: 2.065
Extracellular matrix turnover in coronary artery ectasia patients

DOI: 10.1007/s00380-014-0622-4

Sample: Serum
CLINICAL RHEUMATOLOGY (2019) IF: 2.293
Associations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis

DOI: 10.1007/s10067-019-04718-8

PMID: 31377918
Sample: Serum
CLINICAL BIOCHEMISTRY (2016) IF: 2.382
Circulating biomarkers of fibrosis and cardioversion of atrial fibrillation: A prospective, controlled cohort study

DOI: 10.1016/j.clinbiochem.2016.09.008

Sample: Serum
PLoS One (2018) IF: 2.766
Left atrial voltage, circulating biomarkers of fibrosis, and atrial fibrillation ablation. A prospective cohort study

DOI: 10.1371/journal.pone.0189936

Sample: serum
CARDIOLOGY (2019) IF: 1.561
Monocyte Chemoattractant Protein-1-Induced Protein in Age-Related Atrial Fibrillation and Its Association with Circulating Fibrosis Biomarkers

DOI: 10.1159/000499932

Sample: serum

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Reviews/Q&A

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Reviews
Q&A

A*******aSubmitted [ Apr 19 2021 ]

Asked: Can we get coated plates separately for P3NP kit (cat.no. E-EL-H0183)

adminSubmitted [ Apr 19 2021 ]

Answered: Dear Aishwarya, Thanks for your inquiry. I just have replied to your email box, pls kindly check it out. Thank you and best regards.

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