Human PⅢNP (N-Terminal Procollagen Ⅲ Propeptide) CLIA Kit (E-CL-H0167)

Name: Human PⅢNP (N-Terminal Procollagen Ⅲ Propeptide) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0167
Price: 180.0 USD
Availability: InStock

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Human PⅢNP (N-Terminal Procollagen Ⅲ Propeptide) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PⅢNP in samples.No significant cross-reactivity or interference between Human PⅢNP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	55532.000	59640.000	57586.000	57565
400	23213.000	24265.000	23739.000	23718
200	10327.000	10071.000	10199.000	10178
100	4707.000	5241.000	4974.000	4953
50	2520.000	2220.000	2370.000	2349
25	1163.000	999.000	1081.000	1060
12.5	525.000	559.000	542.000	521
0	21.000	21.000	21.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	38.900	72.650	396.540	40.810	77.700	365.700
Standard deviation	4.220	6.840	42.870	3.860	5.940	28.960
CV（%）	10.850	9.420	10.810	9.460	7.640	7.920

Recovery

The recovery of Human PⅢNP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-101	92
EDTA plasma （n=8）	95-107	101
Cell culture media （n=8）	98-116	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PⅢNP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-115	94-107	87-103
1:2	Average	106	102	94
1:4	Range	88-102	96-112	86-96
1:4	Average	94	103	91
1:8	Range	96-112	89-101	93-110
1:8	Average	104	95	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PⅢNP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅢNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅢNP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PⅢNP. You can calculate the concentration of Human PⅢNP in the samples by comparing the RLU value of the samples to the standard curve.

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