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Human RETN(Resistin) ELISA Kit

Citations(14)Uniprot : Q9HD89

Cat.No.:E-EL-H1213
Reactivity: Human

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96T
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human RETN concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RETN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RETN. You can calculate the concentration of Human RETN in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RETN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RETN were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	104.60	212.00	977.10	112.00	217.50	915.30
Standard deviation	7.10	11.90	44.90	7.70	12.20	33.90
CV (%)	6.79	5.61	4.60	6.88	5.61	3.70

Recovery

The recovery of Human RETN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-102	96
EDTA plasma (n=8)	87-101	93
Cell culture media (n=8)	91-107	98

Linearity

Samples were spiked with high concentrations of Human RETN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9HD89
Synonyms	ADSF, FIZZ3, RETN1, RSTN, XCP1
Research Area	Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Endocrinology (2022) IF: 6.055
Serum Resistin Levels and Related Genetic Variants Are Associated With Bone Mineral Density in Postmenopausal Women

DOI: 10.3389/fendo.2022.868120

PMID: 35992125
Sample: serum
Oncotarget (2016) IF: 5.008
Adipocytes secreted leptin is a pro-tumor factor for survival of multiple myeloma under chemotherapy

DOI: 10.18632/oncotarget.13342

Sample: plasma
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation

DOI: 10.1111/jcmm.16275

PMID: 33448094
Sample: Plasma
Frontiers in Endocrinology (2021) IF: 5.555
Association Between Vitamin D and Resistin in Postmenopausal Females With Altered Bone Health

DOI: 10.3389/fendo.2020.615440

Sample: Serum
Scientific Reports (2021) IF: 4.38
Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3

PMID: 33859244
Sample: Plasma
DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

DOI: 10.1016/j.diabres.2019.05.023

PMID: 31150725
Sample: Serum
JOURNAL OF NEPHROLOGY (2021) IF: 3.902
Body composition, adipokines, FGF23-Klotho and bone in kidney transplantation: Is there a link?

DOI: 10.1007/s40620-021-00972-9

PMID: 33560479
Sample: Serum
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY (2020) IF: 2.13
Circadian rhythm abnormalities in patients with inflammatory bowel disease – association with adipokine profile

DOI: 10.1080/00365521.2020.1737727

Sample: Serum
Obesity Research & Clinical Practice (2022) IF: 2.288
The effect of omentectomy on the blood levels of adipokines in obese patients with endometrial cancer

DOI: 10.1016/j.orcp.2022.06.002

PMID: 35701296
Sample: serum
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2020) IF: 2.644
Resistin is not a useful insulin resistance marker for non-obese patients.

DOI: 10.26402/jpp.2020.3.06

Sample: blood
Lipids in Health and Disease (2019) IF: 2.651
Leptin, resistin and visfatin as useful predictors of gestational diabetes mellitus

DOI: 10.1186/s12944-019-1169-2

PMID: 31836012
Sample: Serum
Endokrynologia Polska (2021) IF: 1.582
New insights into the metabolic-bone crosstalk in active acromegaly

DOI: 10.5603/EP.a2021.0028

Sample: serum
Clinical Hypertension (2020)
Serum adipocytokines and adiposity as predictive indices of preeclampsia

DOI: 10.1186/s40885-020-00152-0

PMID: 33014422
Sample: Serum,Urine
Health Care for Women International (2020) IF: 0.97
Serum resistin levels as predictor of low bone mineral density in postmenopausal women

DOI: 10.1080/07399332.2020.1798965

Sample: serum

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