Human S100A11 (S100 Calcium Binding Protein A11) CLIA Kit (E-CL-H0843)

Name: Human S100A11 (S100 Calcium Binding Protein A11) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0843
Price: 180.0 USD
Availability: InStock

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Human S100A11 (S100 Calcium Binding Protein A11) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human S100A11 in samples.No significant cross-reactivity or interference between Human S100A11 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	56747.000	66201.000	61474.000	61434
2000	28827.000	32215.000	30521.000	30481
1000	14569.000	14197.000	14383.000	14343
500	6763.000	7051.000	6907.000	6867
250	3638.000	3428.000	3533.000	3493
125	1839.000	1633.000	1736.000	1696
62.5	769.000	821.000	795.000	755
0	39.000	41.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	218.130	537.350	1645.330	238.260	510.960	1712.520
Standard deviation	21.970	56.800	148.410	27.230	40.060	132.720
CV（%）	10.070	10.570	9.020	11.430	7.840	7.750

Recovery

The recovery of Human S100A11 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-109	102
EDTA plasma （n=8）	91-106	99
Cell culture media （n=8）	93-107	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human S100A11 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-99	85-97	99-115
1:2	Average	91	92	105
1:4	Range	93-103	94-107	95-108
1:4	Average	98	101	101
1:8	Range	94-108	86-102	93-109
1:8	Average	102	93	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A11. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A11, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human S100A11. You can calculate the concentration of Human S100A11 in the samples by comparing the RLU value of the samples to the standard curve.

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