Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit (E-CL-H0844)

Name: Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0844
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human S100A12 in samples.No significant cross-reactivity or interference between Human S100A12 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53502.000	62766.000	58134.000	58101
1000	23866.000	27274.000	25570.000	25537
500	11926.000	11838.000	11882.000	11849
250	5453.000	6133.000	5793.000	5760
125	2899.000	2471.000	2685.000	2652
62.5	1455.000	1295.000	1375.000	1342
31.25	721.000	737.000	729.000	696
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	99.790	307.750	970.140	102.810	336.890	1042.150
Standard deviation	8.500	21.880	71.500	9.580	38.740	113.800
CV（%）	8.520	7.110	7.370	9.320	11.500	10.920

Recovery

The recovery of Human S100A12 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	94
EDTA plasma （n=8）	98-113	105
Cell culture media （n=8）	94-106	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human S100A12 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-103	98-111	89-104
1:2	Average	96	105	97
1:4	Range	97-112	85-96	93-108
1:4	Average	105	91	99
1:8	Range	102-115	86-100	93-106
1:8	Average	108	92	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A12. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A12, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human S100A12. You can calculate the concentration of Human S100A12 in the samples by comparing the RLU value of the samples to the standard curve.

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