Human S100B (S100 Calcium Binding Protein B) CLIA Kit (E-CL-H0848)

Name: Human S100B (S100 Calcium Binding Protein B) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0848
Price: 180.0 USD
Availability: InStock

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Human S100B (S100 Calcium Binding Protein B) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human S100B in samples.No significant cross-reactivity or interference between Human S100B and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	54279.000	60725.000	57502.000	57473
500	22506.000	23432.000	22969.000	22940
250	10350.000	10258.000	10304.000	10275
125	4674.000	5584.000	5129.000	5100
62.5	2492.000	2428.000	2460.000	2431
31.25	1437.000	1243.000	1340.000	1311
15.63	717.000	817.000	767.000	738
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	51.860	97.330	353.430	48.090	100.170	365.560
Standard deviation	4.450	10.450	31.700	3.970	8.450	22.190
CV（%）	8.580	10.740	8.970	8.260	8.440	6.070

Recovery

The recovery of Human S100B was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-109	100
EDTA plasma （n=8）	88-102	95
Cell culture media （n=8）	91-106	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human S100B and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-102	95-108	94-110
1:2	Average	94	102	102
1:4	Range	100-114	93-107	97-113
1:4	Average	107	99	105
1:8	Range	93-104	85-98	85-97
1:8	Average	98	90	92

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the RLU value of the samples to the standard curve.

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