Human SEMA3A (Semaphorin 3A) CLIA Kit (E-CL-H0829)

Name: Human SEMA3A (Semaphorin 3A) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0829
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human SEMA3A (Semaphorin 3A) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human SEMA3A in samples.No significant cross-reactivity or interference between Human SEMA3A and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53701.000	60205.000	56953.000	56913
1000	22703.000	26293.000	24498.000	24458
500	12640.000	11186.000	11913.000	11873
250	5516.000	5870.000	5693.000	5653
125	2696.000	2586.000	2641.000	2601
62.5	1399.000	1355.000	1377.000	1337
31.25	677.000	753.000	715.000	675
0	38.000	42.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	100.040	315.470	856.960	107.940	314.580	772.720
Standard deviation	10.870	23.440	81.670	12.730	34.350	55.330
CV（%）	10.870	7.430	9.530	11.790	10.920	7.160

Recovery

The recovery of Human SEMA3A was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-101	93
EDTA plasma （n=8）	89-102	94
Cell culture media （n=8）	94-108	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human SEMA3A and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-100	89-102	94-109
1:2	Average	93	94	101
1:4	Range	94-109	98-113	95-111
1:4	Average	100	103	102
1:8	Range	92-105	89-99	93-107
1:8	Average	99	94	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA3A. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA3A, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human SEMA3A. You can calculate the concentration of Human SEMA3A in the samples by comparing the RLU value of the samples to the standard curve.

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