Human SNCα (Synuclein Alpha) CLIA Kit (E-CL-H0663)

Name: Human SNCα (Synuclein Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0663
Price: 180.0 USD
Availability: InStock

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Human SNCα (Synuclein Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human SNCα in samples.No significant cross-reactivity or interference between Human SNCα and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	49625.000	60193.000	54909.000	54876
400	22512.000	22636.000	22574.000	22541
200	10910.000	9526.000	10218.000	10185
100	4341.000	5029.000	4685.000	4652
50	2356.000	2242.000	2299.000	2266
25	1207.000	1079.000	1143.000	1110
12.5	545.000	569.000	557.000	524
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	36.550	71.160	322.940	39.240	70.030	319.910
Standard deviation	3.960	7.180	31.290	4.670	6.140	34.130
CV（%）	10.830	10.090	9.690	11.900	8.770	10.670

Recovery

The recovery of Human SNCα was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-98	93
EDTA plasma （n=8）	96-108	101
Cell culture media （n=8）	86-100	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human SNCα and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-117	92-105	95-110
1:2	Average	107	100	101
1:4	Range	88-103	101-118	89-104
1:4	Average	95	108	96
1:8	Range	90-104	96-110	92-103
1:8	Average	95	103	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SNCα. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SNCα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SNCα, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human SNCα. You can calculate the concentration of Human SNCα in the samples by comparing the RLU value of the samples to the standard curve.

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