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Human TE(Telomerase) ELISA Kit

Citations(6)Uniprot : O14746

Cat.No.:E-EL-H0164
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human TE in samples. No significant cross-reactivity or interference between Human TE and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human TE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TE. You can calculate the concentration of Human TE in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TE were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.55	1.53	4.01	0.58	1.50	4.23
Standard deviation	0.03	0.07	0.20	0.04	0.08	0.15
CV (%)	5.45	4.58	4.99	6.90	5.33	3.55

Recovery

The recovery of Human TE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-102	95
EDTA plasma (n=8)	89-105	96
Cell culture media (n=8)	89-103	94

Linearity

Samples were spiked with high concentrations of Human TE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: O14746
Research Area	Cancer, Stem cells, Immunology, Metabolism, Cell Biology, Cardiovascular, Signal transduction, Neuroscience, Tags and Cell Markers, Epigenetics and Nuclear Signal

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
Ganoderic Acid D Protects Human Amniotic Mesenchymal Stem Cells against Oxidative Stress-Induced Senescence through the PERK/NRF2 Signaling Pathway

DOI: 10.1155/2020/8291413

PMID: 32774686
Sample: Cell lysate
BURNS (2018) IF: 2.134
Effects of high-voltage electrical burns and other burns on levels of serum oxidative stress and telomerase in children

DOI: 10.1016/j.burns.2018.07.001

Sample: Serum
Frontiers in Psychology (2022) IF: 2.988
Impact of Meditation-Based Lifestyle Practices on Mindfulness, Wellbeing, and Plasma Telomerase Levels: A Case-Control Study.

DOI: 10.3389/fpsyg.2022.846085

Sample: plasma
JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2021) IF: 1.246
Investigation of the role of serum telomerase levels in patients with occult primary ovarian insufficiency: a prospective cross-sectional study

DOI: 10.1080/01443615.2021.1916807

PMID: 34219577
Sample: serum
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY (2021)
Prognostic significance of serum telomerase activity in the monitoring of hepatitis B viral infection

DOI: 10.1080/15321819.2021.2004162

Sample: serum
Diabetes & Metabolic Syndrome-Clinical Research & Reviews (2019)
Assessment of autonomic functions and its association with telomerase level, oxidative stress and inflammation in complete glycemic spectrum– an exploratory study

DOI: 10.1016/j.dsx.2019.01.011

Sample: Plasma

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