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Human TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit

Name: Human TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
Brand: Elabscience
SKU: E-EL-H0184
Price: 495 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(15)Uniprot : P01033

Cat.No.:E-EL-H0184
Reactivity: Human

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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human TIMP-1 in samples. No significant cross-reactivity or interference between Human TIMP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human TIMP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-1. You can calculate the concentration of Human TIMP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TIMP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TIMP-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.40	3.60	0.50	1.40	3.50
Standard deviation	0.03	0.07	0.11	0.03	0.06	0.11
CV (%)	6.00	5.00	3.06	6.00	4.29	3.14

Recovery

The recovery of Human TIMP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	99
EDTA plasma (n=8)	89-102	95
Cell culture media (n=8)	93-107	99

Linearity

Samples were spiked with high concentrations of Human TIMP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01033
Synonyms	TIMP1, CLGI, EPA, EPO, HCI
Research Area	Cancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

CLINICAL CANCER RESEARCH (2019) IF: 8.911
Tumor-Contacted Neutrophils Promote Metastasis by a CD90-TIMP-1 Juxtacrine–Paracrine Loop

DOI: 10.1158/1078-0432.CCR-18-2544

Sample: tumor-infiltrating neutrophils (TINs),neutrophils
BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis

DOI: 10.1016/j.biopha.2023.114289

Sample: cardiac fibroblast
Antioxidants (2022) IF: 7.675
Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from Sea buckthorn on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts

DOI: 10.3390/antiox11101900

PMID: 36290623
Sample: HSFs
Cells (2021) IF: 6.6
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases

DOI: 10.3390/cells10123506

Sample: cardiac fibroblast
Frontiers in Immunology (2020) IF: 5.085
Infection of Mycobacterium tuberculosis Promotes Both M1/M2 Polarization and MMP Production in Cigarette Smoke-Exposed Macrophages

DOI: 10.3389/fimmu.2020.01902

PMID: 32973788
Sample: Serum
CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells

DOI: 10.1016/j.cbi.2021.109737

PMID: 34740599
Sample: HaCaT cells
INDUSTRIAL CROPS AND PRODUCTS (2022) IF: 5.645
Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction

DOI: 10.1016/j.indcrop.2022.115164

Sample: Normal human dermal fibroblasts (NHDF)
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872
Human arsenic exposure and lung function impairment in coal-burning areas in Guizhou, China

DOI: 10.1016/j.ecoenv.2020.110174

Sample: serum
Journal of Functional Foods (2020) IF: 3.701
Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells

DOI: 10.1016/j.jff.2020.104198

Sample: HaCaT cell
Biomed Research International (2020) IF: 2.276
Effects of 12-Week Exercise Program on Enzyme Activity of Serum Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Female Patients with Postmenopausal Osteoporosis: A Randomized Control Study

DOI: 10.1155/2020/9758289

Sample: serum
Experimental and Therapeutic Medicine (2021) IF: 2.447
Mediators of extracellular matrix degradation and inflammation: A new team of possible biomarkers for oral squamous cell carcinoma stage

DOI: 10.3892/etm.2021.10309

PMID: 34194555
Sample: Oral cancer tissue
INTERNATIONAL DENTAL JOURNAL (2022) IF: 2.607
Efficacy of Proanthocyanidins in Nonsurgical Periodontal Therapy

DOI: 10.1016/j.identj.2022.08.006

PMID: 36167610
Sample: saliva samples
BMC Oral Health (2021) IF: 2.757
The effect of conventional versus electronic cigarette use on treatment outcomes of peri-implant disease

DOI: 10.1186/s12903-021-01784-w

Sample: Saliva
ANDROLOGIA (2022) IF: 2.775
Relationship of interleukin-23 with matrix metalloproteinase-9, pentraxin-3, sleep quality and prostate size in benign prostatic hyperplasia

DOI: 10.1111/and.14387

PMID: 35092070
Sample: serum
MEDICINE (2020) IF: 1.552
Dominative role of MMP-14 over MMP-15 in human urinary bladder carcinoma on the basis of its enhanced specific activity

DOI: 10.1097/MD.0000000000019224

PMID: 32049862
Sample: Tissue homogenate

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Verified Customer

h********nSubmitted [ Jun 14 2019 ]

Species:Human
Sample:serum and plasma
Description:The arrival of the Human TIMP-1 ELISA Kit is faster than I’ve expected . My dilution ratio of the serum and plasma is not accurate at first . luckily , I found the correct dilution ratio trying twice . I should have done more dilution ratio to save time at first . but the Kit of Human TIMP-1 is so sample to use . the datasheet of human TIMP-1 kit is useful in detail , and I am very satisfied with the results . I’ll consider the company in future research.

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S***zSubmitted [ Jun 16 2022 ]

Asked: Hello, I will use E-EL-H0184 kit with the cell culture media. Is there any dilution range that you recommend for cell culture media samples??

adminSubmitted [ Jun 16 2022 ]

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