Human TMPRSS2 (Transmembrane Protease, Serine 2) CLIA Kit (E-CL-H0915)

Name: Human TMPRSS2 (Transmembrane Protease, Serine 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0915
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human TMPRSS2 (Transmembrane Protease, Serine 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TMPRSS2 in samples.No significant cross-reactivity or interference between Human TMPRSS2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54165.000	61967.000	58066.000	58029
1000	25085.000	25937.000	25511.000	25474
500	11610.000	11096.000	11353.000	11316
250	5144.000	5914.000	5529.000	5492
125	2684.000	2664.000	2674.000	2637
62.5	1385.000	1359.000	1372.000	1335
31.25	648.000	734.000	691.000	654
0	37.000	37.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	101.730	264.750	807.970	97.980	244.440	824.800
Standard deviation	10.680	20.810	80.960	12.570	18.550	54.110
CV（%）	10.500	7.860	10.020	12.830	7.590	6.560

Recovery

The recovery of Human TMPRSS2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-112	105
EDTA plasma （n=8）	87-98	93
Cell culture media （n=8）	97-108	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TMPRSS2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	101-118	93-108
1:2	Average	101	107	101
1:4	Range	97-113	85-97	90-102
1:4	Average	104	91	96
1:8	Range	87-101	84-96	91-107
1:8	Average	94	91	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TMPRSS2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TMPRSS2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TMPRSS2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TMPRSS2. You can calculate the concentration of Human TMPRSS2 in the samples by comparing the RLU value of the samples to the standard curve.

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