Human TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit (E-CL-H0108)

Name: Human TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0108
Price: 180.0 USD
Availability: InStock

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Human TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TNF-α in samples.No significant cross-reactivity or interference between Human TNF-α and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	51674.000	60026.000	55850.000	55813
250	19548.000	22946.000	21247.000	21210
125	10252.000	9832.000	10042.000	10005
62.5	4416.000	5264.000	4840.000	4803
31.25	2523.000	2195.000	2359.000	2322
15.63	1293.000	1249.000	1271.000	1234
7.81	735.000	819.000	777.000	740
0	37.000	37.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	25.600	58.030	169.010	27.580	54.450	158.500
Standard deviation	2.690	5.500	13.540	2.460	6.400	14.990
CV（%）	10.510	9.480	8.010	8.920	11.750	9.460

Recovery

The recovery of Human TNF-α was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-113	107
EDTA plasma （n=8）	92-103	98
Cell culture media （n=8）	87-101	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TNF-α and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-110	98-112	86-99
1:2	Average	103	105	93
1:4	Range	85-98	101-117	99-111
1:4	Average	90	109	105
1:8	Range	93-106	91-105	90-102
1:8	Average	98	99	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the RLU value of the samples to the standard curve.

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