Human TNKS1 (Tankyrase 1) CLIA Kit (E-CL-H1420)

Name: Human TNKS1 (Tankyrase 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1420
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TNKS1 in samples.No significant cross-reactivity or interference between Human TNKS1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	60093.000	63463.000	61778.000	61747
2000	28531.000	32731.000	30631.000	30600
1000	15810.000	13386.000	14598.000	14567
500	6353.000	7575.000	6964.000	6933
250	3572.000	3474.000	3523.000	3492
125	1675.000	1503.000	1589.000	1558
62.5	814.000	832.000	823.000	792
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	207.480	492.700	1582.580	226.610	495.160	1562.320
Standard deviation	21.660	53.900	106.030	28.710	47.440	124.830
CV（%）	10.440	10.940	6.700	12.670	9.580	7.990

Recovery

The recovery of Human TNKS1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-106	97
EDTA plasma （n=8）	87-100	92
Cell culture media （n=8）	99-113	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TNKS1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-101	103-117	101-115
1:2	Average	93	109	106
1:4	Range	100-118	90-102	93-104
1:4	Average	108	97	98
1:8	Range	98-111	102-117	101-114
1:8	Average	105	108	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNKS1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNKS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNKS1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TNKS1. You can calculate the concentration of Human TNKS1 in the samples by comparing the RLU value of the samples to the standard curve.