Human TP53(Tumor Protein p53) ELISA Kit

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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H0910

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $580

      Reactivity: Human

      Detection Range: 78.13~5000 pg/mL

      Sensitivity: 46.88 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual
      Intended use
      This ELISA kit applies to the in vitro quantitative determination of Human TP53 concentrations in serum, plasma, saliva and other biological fluids.

      Test principle
      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TP53. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TP53. You can calculate the concentration of Human TP53 in the samples by comparing the OD of the samples to the standard curve.


      Assay type

      Sandwich

      Format

      96T

      Assay time

      3.5h

      Reactivity

      Human

      Detection Method

      Colormetric

      Detection Range

      78.13— 5000pg/mL

      Sensitivity

      46.88 pg/mL

      Sample Volume

      100μL

      Sample Type

      Serum, plasma, saliva and other biological fluids

      Specificity

      This kit recognizes in samples Human TP53. No Significant cross-reactivity or interference between Human TP53 and analogues was observed.



      Kit components & Storage


      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received


      Item

      Specifications

      Storage

      Micro ELISA Plate (Dismountable)

      8 wells ×12 strips

      -20, 6 months

      Reference Standard

      2 vials

      Concentrated Biotinylated Detection Ab (100×)

      1 vial, 120 uL

      Concentrated HRP Conjugate (100×)

      1 vial, 120 μL

      -20(shading light), 6 months

      Reference Standard & Sample Diluent

      1 vial, 20 mL

      4, 6 months

      Biotinylated Detection Ab Diluent

      1 vial, 14 mL

      HRP Conjugate Diluent

      1 vial, 14 mL

      Concentrated Wash Buffer (25×)

      1 vial, 30 mL

      Substrate Reagent

      1 vial, 10 mL

      4(shading light)

      Stop Solution

      1 vial, 10 mL

      4

      Plate Sealer

      5 pieces

      Product Description

      1 copy

      Certificate of Analysis

      1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

              The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring 

              equipment instead of directly pouring into the vial(s).


      Other supplies required


      Microplate reader with 450 nm wavelength 

      filter High-precision transfer pipette, EP tubes and disposable pipette tips 

      Incubator capable of maintaining 37℃ 

      Deionized or distilled water 

      Absorbent paper 

      Loading slot for Wash Buffer




      Citations

      1. Publication: Rohmaniar P D, Berniyanti T, Rahayu R P. The Correlation Between The Use Of Personal Protective Equipment And Level Wild-Type P53 Of Dental Technicians In Surabaya[J]. Dental Journal , 2017, 50(1): 19-22.
        Sample Type: Saliva
      2. Publication: Turacli I D, Candar T, Yuksel E B, et al. Role Of Metformin On Base Excision Repair Pathway In p53 Wild-Type H2009 And HepG2 Cancer Cells[J]. Human & Experimental Toxicology, 2017.
        Sample Type: cell lysis
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