Human TP53 (Tumor Protein p53) CLIA Kit (E-CL-H0621)

Name: Human TP53 (Tumor Protein p53) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0621
Price: 180.0 USD
Availability: InStock

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Human TP53 (Tumor Protein p53) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TP53 in samples.No significant cross-reactivity or interference between Human TP53 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	51497.000	58161.000	54829.000	54803
500	22569.000	22661.000	22615.000	22589
250	11065.000	9867.000	10466.000	10440
125	4931.000	4997.000	4964.000	4938
62.5	2822.000	2568.000	2695.000	2669
31.25	1419.000	1249.000	1334.000	1308
15.63	711.000	733.000	722.000	696
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	51.400	158.820	437.930	47.380	166.340	428.030
Standard deviation	5.470	13.660	48.080	5.990	19.730	45.760
CV（%）	10.640	8.600	10.980	12.640	11.860	10.690

Recovery

The recovery of Human TP53 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-97	92
EDTA plasma （n=8）	85-98	92
Cell culture media （n=8）	96-108	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TP53 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-115	90-102	103-116
1:2	Average	106	96	110
1:4	Range	102-118	86-102	102-116
1:4	Average	109	93	108
1:8	Range	91-104	97-111	97-109
1:8	Average	97	103	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TP53. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TP53. You can calculate the concentration of Human TP53 in the samples by comparing the RLU value of the samples to the standard curve.

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