Human TPS (Tissue Polypeptide Specific Antigen) CLIA Kit (E-CL-H1374)

Name: Human TPS (Tissue Polypeptide Specific Antigen) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1374
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human TPS (Tissue Polypeptide Specific Antigen) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TPS in samples.No significant cross-reactivity or interference between Human TPS and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55734.000	61734.000	58734.000	58692
1000	23999.000	24095.000	24047.000	24005
500	11490.000	10990.000	11240.000	11198
250	5519.000	5905.000	5712.000	5670
125	2804.000	2464.000	2634.000	2592
62.5	1330.000	1324.000	1327.000	1285
31.25	700.000	706.000	703.000	661
0	41.000	43.000	42.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.030	296.850	855.770	93.730	312.770	936.710
Standard deviation	8.220	26.240	69.830	10.520	33.150	61.540
CV（%）	8.740	8.840	8.160	11.220	10.600	6.570

Recovery

The recovery of Human TPS was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-115	107
EDTA plasma （n=8）	90-105	97
Cell culture media （n=8）	93-107	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TPS and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	87-102	96-113
1:2	Average	98	93	104
1:4	Range	97-111	86-98	102-120
1:4	Average	102	91	109
1:8	Range	91-103	99-115	88-99
1:8	Average	97	107	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TPS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TPS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TPS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TPS. You can calculate the concentration of Human TPS in the samples by comparing the RLU value of the samples to the standard curve.

Background