Human Tyk-2 (Tyrosine Kinase 2) CLIA Kit (E-CL-H1048)

Name: Human Tyk-2 (Tyrosine Kinase 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1048
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human Tyk-2 (Tyrosine Kinase 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human Tyk-2 in samples.No significant cross-reactivity or interference between Human Tyk-2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	62582.000	65662.000	64122.000	64095
2000	28924.000	29290.000	29107.000	29080
1000	15394.000	13154.000	14274.000	14247
500	7156.000	7212.000	7184.000	7157
250	3635.000	3485.000	3560.000	3533
125	1671.000	1643.000	1657.000	1630
62.5	771.000	867.000	819.000	792
0	27.000	27.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	218.660	621.650	1720.500	198.930	566.490	1553.570
Standard deviation	23.220	61.610	159.150	25.540	66.850	96.790
CV（%）	10.620	9.910	9.250	12.840	11.800	6.230

Recovery

The recovery of Human Tyk-2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-103	97
EDTA plasma （n=8）	87-99	92
Cell culture media （n=8）	97-111	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human Tyk-2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-114	94-111	97-109
1:2	Average	106	102	103
1:4	Range	93-111	104-116	87-99
1:4	Average	101	110	93
1:8	Range	87-100	91-104	95-109
1:8	Average	94	96	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Tyk-2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Tyk-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Tyk-2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human Tyk-2. You can calculate the concentration of Human Tyk-2 in the samples by comparing the RLU value of the samples to the standard curve.