Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit (E-CL-H0816)

Name: Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0816
Price: 180.0 USD
Availability: InStock

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Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human UBE3A in samples.No significant cross-reactivity or interference between Human UBE3A and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	56996.000	64056.000	60526.000	60500
2000	27744.000	28730.000	28237.000	28211
1000	15217.000	13939.000	14578.000	14552
500	6342.000	7168.000	6755.000	6729
250	3326.000	3214.000	3270.000	3244
125	1653.000	1571.000	1612.000	1586
62.5	790.000	808.000	799.000	773
0	26.000	26.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	186.530	343.310	1638.060	198.040	310.290	1602.450
Standard deviation	18.260	24.070	167.900	20.300	32.080	177.070
CV（%）	9.790	7.010	10.250	10.250	10.340	11.050

Recovery

The recovery of Human UBE3A was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-106	99
EDTA plasma （n=8）	83-99	90
Cell culture media （n=8）	96-113	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human UBE3A and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-114	99-114	103-116
1:2	Average	105	106	110
1:4	Range	99-111	99-112	92-106
1:4	Average	106	106	99
1:8	Range	95-111	102-118	92-104
1:8	Average	103	109	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBE3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE3A, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human UBE3A. You can calculate the concentration of Human UBE3A in the samples by comparing the RLU value of the samples to the standard curve.

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