Human VS (Versican/PG-M) CLIA Kit (E-CL-H0801)

Name: Human VS (Versican/PG-M) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0801
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human VS in samples.No significant cross-reactivity or interference between Human VS and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	56640.000	61996.000	59318.000	59288
2000	26841.000	30697.000	28769.000	28739
1000	14359.000	12929.000	13644.000	13614
500	6409.000	7301.000	6855.000	6825
250	3519.000	3441.000	3480.000	3450
125	1605.000	1585.000	1595.000	1565
62.5	742.000	786.000	764.000	734
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	194.640	410.500	1592.990	189.060	381.410	1510.620
Standard deviation	17.360	32.350	106.570	21.020	35.510	99.550
CV（%）	8.920	7.880	6.690	11.120	9.310	6.590

Recovery

The recovery of Human VS was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	94
EDTA plasma （n=8）	100-116	106
Cell culture media （n=8）	100-114	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human VS and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-117	97-114	102-118
1:2	Average	109	105	109
1:4	Range	85-95	98-113	95-110
1:4	Average	90	104	103
1:8	Range	101-114	88-102	100-114
1:8	Average	108	93	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human VS. You can calculate the concentration of Human VS in the samples by comparing the RLU value of the samples to the standard curve.

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