Human β-EPR (Beta-Endorphin Receptor) CLIA Kit (E-CL-H0445)

Name: Human β-EPR (Beta-Endorphin Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0445
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human β-EPR (Beta-Endorphin Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human β-EPR in samples.No significant cross-reactivity or interference between Human β-EPR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	48975.000	56441.000	52708.000	52682
400	20580.000	24298.000	22439.000	22413
200	10569.000	8905.000	9737.000	9711
100	4548.000	4584.000	4566.000	4540
50	2372.000	2212.000	2292.000	2266
25	1164.000	1120.000	1142.000	1116
12.5	521.000	535.000	528.000	502
0	26.000	26.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	38.400	79.440	328.140	40.390	81.290	301.580
Standard deviation	3.510	6.910	20.380	4.960	7.970	34.410
CV（%）	9.140	8.700	6.210	12.280	9.800	11.410

Recovery

The recovery of Human β-EPR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-102	94
EDTA plasma （n=8）	88-103	95
Cell culture media （n=8）	98-115	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human β-EPR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-101	100-117	97-109
1:2	Average	93	107	102
1:4	Range	90-102	88-100	86-101
1:4	Average	97	93	93
1:8	Range	93-106	96-108	98-113
1:8	Average	100	102	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human β-EPR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human β-EPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human β-EPR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human β-EPR. You can calculate the concentration of Human β-EPR in the samples by comparing the RLU value of the samples to the standard curve.