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Lactate Dehydrogenase (LDH) Activity Assay Kit

  • Cat.No.:E-BC-K046-S

  • Detection instrument: Spectrophotometer (450 nm)

To Purchase E-BC-K046-S

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  • 100 assays
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Detection principle

Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death.

Performance characteristics

Synonyms LDH
Sample type Serum, plasma, tissue, cells
Sensitivity 4 U/L
Detection range 4-400 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 7.200%Average intra-assay CV: 2.200%
Other instruments required Micropipettor, Vortex mixer, Water bath
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4-400 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

10-15

Human plasma

10-15

Porcine serum

20-30

Rat serum

20-30

Rat plasma

20-30

10% Rat liver tissue homogenate

800-1000

10% Mouse kidney tissue homogenate

500-800

10% Rat lung tissue homogenate

300-500

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    Sample: pig testicular cell (ST cell)
  2. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECs

    DOI: 10.1155/2021/9919466

    Sample: HUVECs
  3. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 5.195
    A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

    DOI: 10.1016/j.ejphar.2022.175172

    PMID: 35944619

    Sample: serum
  4. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079

    Sample: livers
  5. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    Sample: serum
  6. Journal of Cardiovascular Translational Research (2022) IF: 4.132
    CD137 Signal Mediates Cardiac Ischemia–Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes

    DOI: 10.1007/s12265-022-10240-1

    Sample: serum,HL-1 cell
  7. AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667
    Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

    DOI: 10.1142/S0192415X21500452

    PMID: 33827384

    Sample: serum
  8. NEUROSCIENCE LETTERS (2021) IF: 3.046
    Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

    DOI: 10.1016/j.neulet.2021.136385

    PMID: 34871743

    Sample: serum
  9. REPRODUCTION (2020) IF: 3.206
    Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

    DOI: 10.1530/REP-20-0381

    PMID: 33112813

    Sample: testis
  10. JOURNAL OF GENE MEDICINE (2020) IF: 3.258
    miR‐129‐5p attenuates hypoxia‐induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy

    DOI: 10.1002/jgm.3200

    Sample: H9c2 cell
  11. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575
    Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

    DOI: 10.1016/j.bbrc.2018.02.121

    Sample: serum
  12. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    Sample: serum
  13. LIFE SCIENCES (2020) IF: 3.647
    Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

    DOI: 10.1016/j.lfs.2020.118118

    Sample: serum
  14. MICROVASCULAR RESEARCH (2023) IF: 3.75
    Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats

    DOI: 10.1016/j.mvr.2023.104497

    Sample: Serum
  15. MOLECULAR BIOLOGY REPORTS (2021) IF: 2.316
    Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease

    DOI: 10.1007/s11033-021-06779-y

    Sample: SH-SY5Y cell
  16. JOURNAL OF BIOMATERIALS APPLICATIONS (2021) IF: 2.646
    Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer:

    DOI: 10.1177/08853282211027889

    Sample: MC3T3-E1 cells
  17. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540

    Sample: hepatic tissue,serum
  18. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603

    Sample: brain
  19. Molecular Medicine Reports (2016) IF: 1.559
    Protective effects of ghrelin against oxidative stress, inducible nitric oxide synthase and inflammation in a mouse model of myocardial ischemia/reperfusion injury via the HMGB1 and TLR4/NF-κB pathway

    DOI: 10.3892/mmr.2016.5535

    Sample: serum
  20. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009

    Sample: plasma
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N************RSubmitted [ Aug 05 2023 ]

Asked: Is this kit appropriate for studying with plants (lichens)?

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A

adminSubmitted [ Aug 05 2023 ]

Answered: We'll reply to you by email. Pls pay attention to the mailbox

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