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Lactate Dehydrogenase (LDH) Activity Assay Kit

Cat.No.:E-BC-K046-S
Detection instrument: Spectrophotometer (450 nm)

Detection method: Colorimetric method

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100 assays

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Detection principle

Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death.

Performance characteristics

Synonyms	LDH
Sample type	Serum, plasma, tissue, cells
Sensitivity	4 U/L
Detection range	4-400 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 7.200%Average intra-assay CV: 2.200%
Other instruments required	Micropipettor, Vortex mixer, Water bath
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4-400 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	10-15
Human plasma	10-15
Porcine serum	20-30
Rat serum	20-30
Rat plasma	20-30
10% Rat liver tissue homogenate	800-1000
10% Mouse kidney tissue homogenate	500-800
10% Rat lung tissue homogenate	300-500

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 5.195

A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

DOI: 10.1016/j.ejphar.2022.175172
PMID: 35944619	Sample: Serum

FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025

Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

DOI: 10.1016/j.fct.2022.113061
PMID: 35489465	Sample: Pig Testicular Cell (St Cell)

Toxicology Reports (2019)

Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

DOI: 10.1016/j.toxrep.2019.06.009
	Sample: Plasma

Molecular Medicine Reports (2016) IF: 1.559

Protective effects of ghrelin against oxidative stress, inducible nitric oxide synthase and inflammation in a mouse model of myocardial ischemia/reperfusion injury via the HMGB1 and TLR4/NF-κB pathway

DOI: 10.3892/mmr.2016.5535
	Sample: Serum

MOLECULAR BIOLOGY REPORTS (2021) IF: 2.316

Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease

DOI: 10.1007/s11033-021-06779-y
	Sample: Sh-Sy5Y Cell

JOURNAL OF BIOMATERIALS APPLICATIONS (2021) IF: 2.646

Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer:

DOI: 10.1177/08853282211027889
	Sample: Mc3T3-E1 Cells

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65

San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540
	Sample: Hepatic Tissue,Serum

Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693

Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

DOI: 10.1002/jez.2603
	Sample: Brain

NEUROSCIENCE LETTERS (2021) IF: 3.046

Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385
PMID: 34871743	Sample: Serum

REPRODUCTION (2020) IF: 3.206

Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

DOI: 10.1530/REP-20-0381
PMID: 33112813	Sample: Testis

JOURNAL OF GENE MEDICINE (2020) IF: 3.258

miR‐129‐5p attenuates hypoxia‐induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy

DOI: 10.1002/jgm.3200
	Sample: H9C2 Cell

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575

Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

DOI: 10.1016/j.bbrc.2018.02.121
	Sample: Serum

METABOLIC BRAIN DISEASE (2022) IF: 3.584

Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

DOI: 10.1007/s11011-022-00952-3
PMID: 35298730	Sample: Serum

LIFE SCIENCES (2020) IF: 3.647

Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

DOI: 10.1016/j.lfs.2020.118118
	Sample: Serum

MICROVASCULAR RESEARCH (2023) IF: 3.75

Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats

DOI: 10.1016/j.mvr.2023.104497
	Sample: Serum

Journal of Cardiovascular Translational Research (2022) IF: 4.132

CD137 Signal Mediates Cardiac Ischemia–Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes

DOI: 10.1007/s12265-022-10240-1
	Sample: Serum,Hl-1 Cell

AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667

Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

DOI: 10.1142/S0192415X21500452
PMID: 33827384	Sample: Serum

Food Bioscience (2022) IF: 5.318

Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

DOI: 10.1016/j.fbio.2022.102079
	Sample: Livers

Foods (2022) IF: 5.561

Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

DOI: 10.3390/foods11193048
PMID: 36230124	Sample: Serum

Oxidative Medicine and Cellular Longevity (2021) IF: 6.543

Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECs

DOI: 10.1155/2021/9919466
	Sample: Huvecs

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N************RSubmitted [ Aug 05 2023 ]

Asked: Is this kit appropriate for studying with plants (lichens)?

adminSubmitted [ Aug 05 2023 ]

Answered: We'll reply to you by email. Pls pay attention to the mailbox

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