Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
Price: $ 220
Price: $ 150
- Sample Type: serum;plasma;animal tissue
- Detection Instrument: Microplate reader(530-540 nm,optimum wavelength: 532 nm)
- Reasearch Areas: Oxidative stress;Ferroptosis
For research use only. Order now, ship in 3 days
Detection Principle | MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm. | ||||||||||||||||||||||||||
Synonyms | MDA | ||||||||||||||||||||||||||
Sample Type | serum,plasma,animal tissue | ||||||||||||||||||||||||||
Sensitivity | 1.13 μmol/L | ||||||||||||||||||||||||||
Detection Range | 2.92-40 μmol/L | ||||||||||||||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||||||||||||||
Assay Time | 65 min | ||||||||||||||||||||||||||
Precision | inter-assay CV: 7.2% | intra-assay CV: 4.1% | ||||||||||||||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol | ||||||||||||||||||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||||||||||
Images | |||||||||||||||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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Q1:What is the function of the control well? Is it essential?
The control well is mainly set to eliminate the interference of the sample itself. If there is hemolysis or lipemia in the blood sample, it is necessary to set the control well; if there is no such phenomenon, it is not necessary to set the control well.
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Q2:Can the 100 掳 C water bath mentioned in the manual be replaced by a metal bath?
Yes, as long as the temperature can reach 100℃ and the heat is uniform.