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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

  • Cat.No.:E-BC-K025-M

  • Detection instrument: Microplate reader(530-540 nm,optimum wavelength: 532 nm)

To Purchase E-BC-K025-M

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  • 48T
Price: $220
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Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms MDA
Sample type Serum,plasma,animal tissue
Sensitivity 1.13 μmol/L
Detection range 2.92-40 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 65 min
Precision Average inter-assay CV: 7.2%Average intra-assay CV: 4.1%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (2.92-40 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Rat serum

1

Rat plasma

1

Mouse serum

1

Mouse plasma

1

10% Rat heart tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat spleen tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat brain tissue homogenate

1


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Veterinary World (2020)
    Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

    DOI: 10.14202/vetworld.2020.1404-1409

    PMID: 32848317

    Sample: Serum
  2. Clinical Cancer Drugs (2019)
    Coenzyme Q10 Attenuates Cisplatin-induced Nephrotoxicity Through Counteracting Oxidative Stress and Inflammation

    DOI: 10.2174/2212697X06666190701113043

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  3. International Journal of Sciences: Basic and Applied Research (2016)
    Effects of Red Dragon Fruits (Hylocereus polyrhizus) Powder and Swimming Exercise on Inflammation, Oxidative Stress Markers, and Physical Fitness in Male Obesity Rats (Sprague dawley)

    PMID: 99999999

    Sample: Plasma
  4. Journal of Biochemical Technology (2020)
    The Hepatoprotective Effect of Moringa Oleifera Leaf Aqueous Extract Against Cadmium-Induced Toxicity on Male Rats

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  5. Journal of International Pharmaceutical Research (2019)
    Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits

    PMID: 99999999

    Sample: Serum
  6. Indian Journal of Animal Research (2019) IF: 0.395
    Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study

    DOI: 10.18805/ijar.B-1157

    PMID: 99999999

    Sample: Tissue homogenate
  7. Synergy (2018)
    Omega-3 fatty acids moderate oxidative and proinflammatory events in experimental hepatotoxicity in Wistar rats: comparison with livolin

    DOI: 10.1016/j.synres.2018.08.001

    PMID: 99999999

    Sample: Plasma
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