MDA(Malondialdehyde) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
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    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-0060

      Synonyms:Malonic aldehyde; Malonodialdehyde; Propanedial; 1,3-Propanedial ; Malonaldehyde

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 31.25~2000 ng/mL

      Sensitivity: 18.75 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of  MDA concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MDA. During the reaction,  MDA in the sample or standard competes with a fixed amount of MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  MDA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  MDA in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type Competitive-ELISA
      Format 96T
      Assay time 2.5h
      Reactivity Universal
      Detection Method Colormetric
      Detection Range 31.25—2000 ng/mL
      Sensitivity 18.75 ng/mL
      Sample Volume 50μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes MDA in samples. No significant cross-reactivity or interference between MDA and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(ng/mL) O.D Average
      2000 0.352
      0.398
      0.375
      1000 0.498
      0.508
      0.503
      500 0.741
      0.709
      0.725
      250 1.058
      1.068
      1.063
      125 1.507
      1.481
      1.494
      62.5 1.942
      1.92
      1.931
      31.25 2.284
      2.284
      2.284
      0 2.804
      2.818
      2.811

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MDA were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MDA were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 109.60 308.70 848.80 111.50 321.00 892.50
      Standard deviation 6.10 17.30 37.30 7.00 17.00 46.40
      C V (%) 5.57 5.60 4.39 6.28 5.30 5.20

      Recovery

      The recovery of  MDA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 92-103 97
      EDTA plasma (n=5) 94-107 99
      Cell culture media (n=5) 93-110 100

      Linearity

      Samples were spiked with high concentrations of MDA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 92-107 84-99 89-101
      Average (%) 99 91 95
      1:4 Range (%) 82-95 88-103 100-113
      Average (%) 89 95 105
      1:8 Range (%) 86-97 87-102 99-111
      Average (%) 92 94 105
      1:16 Range (%) 85-96 87-99 94-110
      Average (%) 91 92 100

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      1.Add 50 μL standard or sample to each well.

      2.Immediately add 50 μL Biotinylated Detection Ab to each well.

      3.Incubate for 45 min at 37℃. Aspirate and wash 3 times.

      4.Add 100 μL HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      5.Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.

      6.Add 50 μL Stop Solution.

      7.Read at 450 nm immediately. Calculation of results.

      Citations

      1. Publication: Zhao J, Xu S Z, Song F, et al. 2, 3, 5, 4' -Tetrahydroxystilbene-2-O-β-D-Glucoside Protects Human Umbilical Vein Endothelial Cells Against Lysophosphatidylcholine-Induced Apoptosis By Upregulating Superoxide Dismutase And Glutathione Peroxidase[J]. Iubmb Life, 2014, 66(10): 711-722.
        Sample Type: Cell lysis
      2. Publication: Cheng Q, Sun G J, Liu S B, et al. A Novel Translocator Protein 18 kDa Ligand, ZBD-2, Exerts Neuroprotective Effects Against Acute Spinal Cord Injury[J]. Clinical and Experimental Pharmacology and Physiology, 2016, 43(10): 930-938.
        Sample Type: Serum
      3. Publication: Luo Y, Fu C, Wang Z, et al. Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And Anti‑Inflammatory Effects, And Inhibition Of The p38‑MAPK Mechanism[J]. Molecular Medicine Reports, 2015, 12: 8294-8300.
        Sample Type: Serum
      4. Publication: Tan C, Lu S, Wang Y, et al. Long-Term Exposure To High Air Pollution Induces Cumulative DNA Damages In Traffic Policemen[J]. Science of The Total Environment, 2017, 593: 330-336.
        Sample Type: Serum
      5. Publication: Jeong H, Liu Y, Kim H S. Dried Plum And Chokeberry Ameliorate D-Galactose-Induced Aging In Mice By Regulation Of Pl3K/Akt-Mediated Nrf2 And Nf-kB Pathways[J]. Experimental Gerontology, 2017, 95: 16-25.
        Sample Type: Serum
      6. Publication: Kızılay Z, Kahraman Çetin N, Aksel M, et al. Ozone Partially Decreases Axonal And Myelin Damage In An Experimental Sciatic Nerve Injury Model[J]. Journal of Investigative Surgery, 2017.
        Sample Type: Serum
      7. Publication: Kim J, Choung S Y. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups[J]. Molecular Vision, 2017, 23: 638-648.
        Sample Type: Tissue homogenate
      8. Publication: Bai D, Jin G, Yin S, et al. Antioxidative And Anti-Apoptotic Roles Of Silibinin In Reversing Learning And Memory Deficits In APP/PS1 Mice[J]. Neurochemical Research, 2017: 1-7.
        Sample Type: Tissue homogenate
      9. Publication: Su H M, Feng L N, Zheng X D, et al. Myricetin Protects Against Diet-Induced Obesity And Ameliorates Oxidative Stress In C57BL/6 Mice[J]. Journal of Zhejiang University-Science B, 2016, 17(6): 437-446.
        Sample Type: Serum
      10. Publication: Franceschelli S, Gatta D M P, Pesce M, et al. Modulation of the oxidative plasmatic state in gastroesophageal reflux disease with the addition of rich water molecular hydrogen: A new biological vision[J]. Journal of Cellular and Molecular Medicine, 2018.
      11. Publication: Bahadır A, Ceyhan A, Gergin Ö Ö, et al. Protective Effects of Curcumin and Beta-carotene on Cisplatin-induced Cardiotoxicity: An Experimental Rat Model[J]. Anatolian Journal of Cardiology, 2018, 19(3): 213.
      12. Publication: Oladosu O W, Biliaminu S A, Abdulazeez I M, et al. Assessment of seminal biomarker of lipid peroxidation among male partners of infertile couples at the university of ilorin teaching hospital, Nigeria[J]. Nigerian Postgraduate Medical Journal, 2018, 25(2): 94.
        Sample Type: Semen
      13. Publication: Guo X, Fan Y, Cui J, et al. NOX4 expression and distal arteriolar remodeling correlate with pulmonary hypertension in COPD[J]. BMC pulmonary medicine, 2018, 18(1): 111.
        Sample Type: Human
      14. Publication: SONG Quan Quan, NIU Jing Ping, ZHANG Shu Yu, LIANG Ting Ting, ZHOU Ji, FENG Shan Shan. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice. Biomedical and Environmental Sciences, 2018, 31(10): 757-768.
        Sample Type: Mouse
      15. Publication: Peng Zeng, Yan Shi, Xiao-Ming Wang, Li Lin, Yan-Jun Du, Na Tang, Qun Wang, Ying-Yan Fang, Jian-Zhi Wang, Xin-Wen Zhou, Youming Lu, Qing Tian; Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats, International Journal of Neuropsychopharmacology, pyy090.
        Sample Type: Rat
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