MEL (Melamine) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E010

      Size:
      • 96T
      Qty:
      - +
      Price: $200

      sample_type: Milk powder,Milk,Tissue,Liver,Feed,Eggss,Serum

      Sensitivity: 2 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Melamine (MEL) in samples, such as milk, tissue, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, MEL in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-MEL antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of MEL. The concentration of MEL in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 2 ppb (ng/mL)

      Reaction mode: 25, 30 min~15 min

      Detection limit: Milk powder---40 ppb; Milk---54 ppb; Milk, Milk powder (method 2) ---2 ppb; Feed---200 ppb

                                Tissue (livestock, fish, shrimp), Liver ---4 ppb; Eggs---40 ppb; Serum---8 ppb

      Cross-reactivity: Melamine ---100%, Cyanuric Acid---60%, s-Triazine1%

      Sample recovery rate:Milk powder, Milk---90±20%; Tissue---85±20%; Feed---85±20%; Eggs, Serum---80±20%

                  

      Kits components

       

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb,2 ppb,6 ppb,18 ppb,54 ppb, 162 ppb)

      HRP Conjugate 

      5.5 mL

      Antibody Working Solution 

      5.5 mL

      Substrate Reagent A 

      6 mL

      Substrate Reagent B 

      6 mL

      Stop Solution 

      6 mL

      20×Concentrated Wash Buffer 

      40 mL

      2×Reconstitution Buffer 

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other materials required but not supplied

      Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: N-hexane, Acetonitrile, NaOH, Concentrated HCl, Methanol.


      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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