Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit (E-BC-K149-M)
Add to cart
For research use only.
| Detection Principle | Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The activity of NADH can be reflected by measuring the absorbance decline rate at 340 nm. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Mitochondria Function , Cuproptosis , Energy Metabolism |
| Other Reagents Required | Anhydrous ethanol (AR) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 4.33 U/L |
| Detection Range | 4.33-224.2 U/L |
| Precision | inter-assay CV: 8.4% | intra-assay CV: 0.8% |
| Sample Volume | 40 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat muscle tissue homogenate | 1-2 |
| 10% Rat lung tissue homogenate | 4-8 |
| 10% Mouse liver tissue homogenate | 1-2 |
| 10% Mouse heart tissue homogenate | 4-8 |
| 10% Rat heart tissue homogenate | 1-2 |
| 10% Rat liver tissue homogenate | 1-2 |
| 10% Rat kidney tissue homogenate | 2-4 |
| 10% Porcine heart tissue homogenate | 1-2 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
| Cat.No. | Product Name | Clone No. |
|---|
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
