Mouse ADP/Acrp30 (Adiponectin) CLIA Kit (E-CL-M0002)

Name: Mouse ADP/Acrp30 (Adiponectin) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0002
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse ADP/Acrp30 (Adiponectin) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse ADP/Acrp30 in samples.No significant cross-reactivity or interference between Mouse ADP/Acrp30 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	29845.000	36151.000	32998.000	32964
5	15702.000	18088.000	16895.000	16861
2.5	7855.000	7211.000	7533.000	7499
1.25	3606.000	3960.000	3783.000	3749
0.63	1960.000	1676.000	1818.000	1784
0.31	918.000	850.000	884.000	850
0.16	353.000	383.000	368.000	334
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.480	0.980	5.000	0.510	1.060	4.840
Standard deviation	0.040	0.080	0.570	0.060	0.120	0.480
CV（%）	8.330	8.160	11.400	11.760	11.320	9.920

Recovery

The recovery of Mouse ADP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-102	96
EDTA plasma （n=8）	93-109	100
Cell culture media （n=8）	89-103	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse ADP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-111	89-104	89-105
1:2	Average	101	97	96
1:4	Range	88-101	88-103	95-111
1:4	Average	94	95	101
1:8	Range	91-103	99-116	84-98
1:8	Average	96	106	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP/Acrp30. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse ADP/Acrp30. You can calculate the concentration of Mouse ADP/Acrp30 in the samples by comparing the RLU value of the samples to the standard curve.

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