Mouse AhR (Aryl Hydrocarbon Receptor) CLIA Kit (E-CL-M0117)

Name: Mouse AhR (Aryl Hydrocarbon Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0117
Price: 180.0 USD
Availability: InStock

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Mouse AhR (Aryl Hydrocarbon Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse AhR in samples.No significant cross-reactivity or interference between Mouse AhR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	58119.000	64977.000	61548.000	61517
2500	32341.000	39023.000	35682.000	35651
1250	19897.000	16945.000	18421.000	18390
625	9303.000	10745.000	10024.000	9993
312.5	5446.000	5110.000	5278.000	5247
156.25	2779.000	2437.000	2608.000	2577
78.13	1340.000	1446.000	1393.000	1362
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	239.730	772.250	2273.220	236.890	830.970	2346.550
Standard deviation	28.260	65.870	218.230	24.780	59.330	269.620
CV（%）	11.790	8.530	9.600	10.460	7.140	11.490

Recovery

The recovery of Mouse AhR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-99	93
EDTA plasma （n=8）	94-105	100
Cell culture media （n=8）	99-112	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse AhR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-109	84-96	100-115
1:2	Average	101	90	107
1:4	Range	102-119	95-109	100-115
1:4	Average	110	102	106
1:8	Range	87-98	99-115	86-98
1:8	Average	92	107	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AhR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AhR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse AhR. You can calculate the concentration of Mouse AhR in the samples by comparing the RLU value of the samples to the standard curve.

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