Mouse ASGR1 (Asialoglycoprotein Receptor 1) CLIA Kit (E-CL-M0119)

Name: Mouse ASGR1 (Asialoglycoprotein Receptor 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0119
Price: 180.0 USD
Availability: InStock

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Mouse ASGR1 (Asialoglycoprotein Receptor 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse ASGR1 in samples.No significant cross-reactivity or interference between Mouse ASGR1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52974.000	55824.000	54399.000	54367
400	22764.000	24884.000	23824.000	23792
200	10993.000	9411.000	10202.000	10170
100	4684.000	5116.000	4900.000	4868
50	2236.000	2144.000	2190.000	2158
25	1179.000	1137.000	1158.000	1126
12.5	485.000	545.000	515.000	483
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	37.970	109.640	386.710	35.550	105.790	415.650
Standard deviation	3.710	9.760	39.830	4.590	12.420	28.390
CV（%）	9.770	8.900	10.300	12.910	11.740	6.830

Recovery

The recovery of Mouse ASGR1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-112	102
EDTA plasma （n=8）	96-110	103
Cell culture media （n=8）	94-108	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse ASGR1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-103	85-100	93-108
1:2	Average	95	91	101
1:4	Range	92-106	101-115	89-101
1:4	Average	100	107	95
1:8	Range	91-104	96-111	103-119
1:8	Average	97	103	110

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ASGR1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ASGR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ASGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse ASGR1. You can calculate the concentration of Mouse ASGR1 in the samples by comparing the RLU value of the samples to the standard curve.

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