Mouse Aβ40(Amyloid Beta 40) CLIA Kit (E-CL-M0059)

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Reactivity	Mouse
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

	OD1	OD2	Mean OD	Corrected OD
1000	50797.000	51751.000	51274.000	51186
500	26660.000	26930.000	26795.000	26707
250	12118.000	11850.000	11984.000	11896
125	5330.000	5338.000	5334.000	5246
62.5	2736.000	2738.000	2737.000	2649
31.25	1431.000	1453.000	1442.000	1354
15.63	768.000	752.000	760.000	672
0	96.000	80.000	88.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（）	49.650	145.890	378.100	45.500	141.880	399.350
Standard deviation	2.810	6.320	20.460	2.530	6.990	19.850
CV（%）	5.660	4.330	5.410	5.550	4.930	4.970

Recovery

The recovery of Mouse Aβ40 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-110	102
EDTA plasma （n=8）	91-106	98
Cell culture media （n=8）	84-97	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse Aβ40 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.