Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit (E-CL-M0146)

Name: Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0146
Price: 180.0 USD
Availability: InStock

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Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse BALP in samples.No significant cross-reactivity or interference between Mouse BALP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	50900.000	61850.000	56375.000	56349
1000	25652.000	26652.000	26152.000	26126
500	12434.000	11148.000	11791.000	11765
250	5127.000	5931.000	5529.000	5503
125	2881.000	2527.000	2704.000	2678
62.5	1479.000	1339.000	1409.000	1383
31.25	687.000	729.000	708.000	682
0	26.000	26.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	96.750	287.520	947.470	100.480	288.620	1014.090
Standard deviation	11.380	28.780	65.940	10.530	23.060	66.420
CV（%）	11.760	10.010	6.960	10.480	7.990	6.550

Recovery

The recovery of Mouse BALP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-112	107
EDTA plasma （n=8）	95-113	103
Cell culture media （n=8）	88-98	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse BALP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	88-104	101-117
1:2	Average	96	95	108
1:4	Range	86-97	102-118	97-112
1:4	Average	92	109	105
1:8	Range	92-106	96-111	90-104
1:8	Average	100	104	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BALP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BALP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BALP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse BALP. You can calculate the concentration of Mouse BALP in the samples by comparing the RLU value of the samples to the standard curve.

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