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Mouse BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

Citations(5)Uniprot : P21237

Cat.No.:E-EL-M0203
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse BDNF in samples. No significant cross-reactivity or interference between Mouse BDNF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse BDNF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BDNF. You can calculate the concentration of Mouse BDNF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse BDNF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse BDNF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	108.30	200.70	906.70	117.20	182.00	864.00
Standard deviation	7.50	9.00	29.00	6.60	7.60	35.40
CV (%)	6.93	4.48	3.20	5.63	4.18	4.10

Recovery

The recovery of Mouse BDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	99
EDTA plasma (n=8)	93-109	100
Cell culture media (n=8)	95-111	101

Linearity

Samples were spiked with high concentrations of Mouse BDNF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P21237
Synonyms	ANON2, BULN2
Research Area	Cancer, Cardiovascular, Metabolism, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Nutrients (2021) IF: 5.717

Administration of Bifidobacterium breve Improves the Brain Function of Aβ1-42-Treated Mice via the Modulation of the Gut Microbiome

DOI: 10.3390/nu13051602
PMID: 34064762	Sample: Tissue homogenate

Food and Chemical Toxicology (2020) IF: 4.679

Memory-enhancing effects of 7,3',4'-trihydroxyisoflavone by regulation of cholinergic function and BDNF signaling pathway in mice

DOI: 10.1016/j.fct.2020.111160
PMID: 31991199	Sample: Tissue homogenate

Brain Research Bulletin (2020) IF: 3.37

Cotinine ameliorates memory and learning impairment in senescent mice

DOI: 10.1016/j.brainresbull.2020.08.010
PMID: 32818583	Sample: Tissue homogenate

International immunopharmacology (2019) IF: 3.118

Purinergic P2X7 receptor blockade mitigates alcohol-induced steatohepatitis and intestinal injury by regulating MEK1/2-ERK1/2 signaling and egr-1 activity

DOI: 10.1016/j.intimp.2018.11.012
PMID: 30445307	Sample: Tissue homogenate

Frontiers in Aging Neuroscience (2018) IF: 3.582

Nicotine Modulates Cognitive Function in D-Galactose-Induced Senescence in Mice.

DOI: 10.3389/fnagi.2018.00194
PMID: 30061821	Sample: Tissue homogenate

Archives of physiology and biochemistry (2018) IF: 1.806

Endoplasmic reticulum stress in the livers of BDNF heterozygous knockout mice

DOI: 10.1080/13813455.2018.1489850
PMID: 30039987	Sample: Tissue homogenate

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