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Mouse BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

  • Cat.No.:E-EL-M0203

  • Reactivity: Mouse

To Purchase E-EL-M0203

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse BDNF in samples. No significant cross-reactivity or interference between Mouse BDNF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse BDNF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BDNF. You can calculate the concentration of Mouse BDNF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse BDNF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse BDNF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 108.30 200.70 906.70 117.20 182.00 864.00
Standard deviation 7.50 9.00 29.00 6.60 7.60 35.40
CV (%) 6.93 4.48 3.20 5.63 4.18 4.10


The recovery of Mouse BDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 99
EDTA plasma (n=8) 93-109 100
Cell culture media (n=8) 95-111 101


Samples were spiked with high concentrations of Mouse BDNF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P21237
SynonymsANON2, BULN2
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nutrients (2021) IF: 5.717
    Administration of Bifidobacterium breve Improves the Brain Function of Aβ1-42-Treated Mice via the Modulation of the Gut Microbiome

    DOI: 10.3390/nu13051602

    PMID: 34064762

    Sample: Tissue homogenate
  2. Food and Chemical Toxicology (2020) IF: 4.679
    Memory-enhancing effects of 7,3',4'-trihydroxyisoflavone by regulation of cholinergic function and BDNF signaling pathway in mice

    DOI: 10.1016/j.fct.2020.111160

    PMID: 31991199

    Sample: Tissue homogenate
  3. Brain Research Bulletin (2020) IF: 3.37
    Cotinine ameliorates memory and learning impairment in senescent mice

    DOI: 10.1016/j.brainresbull.2020.08.010

    PMID: 32818583

    Sample: Tissue homogenate
  4. International immunopharmacology (2019) IF: 3.118
    Purinergic P2X7 receptor blockade mitigates alcohol-induced steatohepatitis and intestinal injury by regulating MEK1/2-ERK1/2 signaling and egr-1 activity

    DOI: 10.1016/j.intimp.2018.11.012

    PMID: 30445307

    Sample: Tissue homogenate
  5. Frontiers in Aging Neuroscience (2018) IF: 3.582
    Nicotine Modulates Cognitive Function in D-Galactose-Induced Senescence in Mice.

    DOI: 10.3389/fnagi.2018.00194

    PMID: 30061821

    Sample: Tissue homogenate
  6. Archives of physiology and biochemistry (2018) IF: 1.806
    Endoplasmic reticulum stress in the livers of BDNF heterozygous knockout mice

    DOI: 10.1080/13813455.2018.1489850

    PMID: 30039987

    Sample: Tissue homogenate
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