Mouse BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit (E-CL-M0142)

Name: Mouse BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0142
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse BMP-4 in samples.No significant cross-reactivity or interference between Mouse BMP-4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52799.000	56173.000	54486.000	54455
400	21232.000	22720.000	21976.000	21945
200	10475.000	9751.000	10113.000	10082
100	4173.000	4971.000	4572.000	4541
50	2473.000	2227.000	2350.000	2319
25	1103.000	1037.000	1070.000	1039
12.5	531.000	573.000	552.000	521
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	43.600	71.550	312.630	47.830	78.440	304.450
Standard deviation	4.760	7.890	32.450	5.810	8.980	26.910
CV（%）	10.920	11.030	10.380	12.150	11.450	8.840

Recovery

The recovery of Mouse BMP-4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-105	96
EDTA plasma （n=8）	100-118	107
Cell culture media （n=8）	93-108	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse BMP-4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-102	101-114	97-115
1:2	Average	96	108	105
1:4	Range	103-117	92-104	101-113
1:4	Average	110	98	107
1:8	Range	100-116	90-102	96-106
1:8	Average	108	96	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse BMP-4. You can calculate the concentration of Mouse BMP-4 in the samples by comparing the RLU value of the samples to the standard curve.

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